Reagentia en instrumenten voor immunologie, celbiologie en moleculaire biologie.
 
> Vimentin (HRP)

Vimentin (HRP)

Afdrukken Afdrukken
Log in alstublieft

Referentie 169222-1ml

Formaat : 1ml

Merk : US Biological


169222 Vimentin (HRP)

Clone Type
Monoclonal
Host
mouse
Source
human
Isotype
IgG1
Grade
Purified
Applications
IC IHC WB
Crossreactivity
Ca Ch Gt Hm Hu Mk Mo Po Rt Ze
Shipping Temp
Blue Ice
Storage Temp
-20°C

Vimentin (57kD) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:1000
Immunohistochemistry (frozen): 1:100-1:200. With avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: RV202|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, 0,1% BSA Labeled with horseradish peroxidase (HRP). Do not add azide.|Purity: Purified|Immunogen: Vimentin extract of bovine lens. |Specificity: Recognizes Vimentin. Species Crossreactivity: Canine, chicken, goat, human, monkey, mouse, rat, porcine, zebrafish. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Vimentin extract of bovine lens.
Form
Supplied as a liquid in PBS, 0,1% BSA Labeled with horseradish peroxidase (HRP). Do not add azide.
Purity
Purified
Specificity
Recognizes Vimentin. Species Crossreactivity: Canine, chicken, goat, human, monkey, mouse, rat, porcine, zebrafish.
References
1. Ramaekers, F., Huysmans, A., Schaart, G., Moesker, O., and Vooijs, P. (1987). Tissue distribution of keratin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49. 2. Viebahn, C., Lane, E. B., and Ramaekers, F. C. (1988). Keratin and vimentin expression in early organogenesis of the rabbit embryo, Cell Tissue Res 253, 553-62. 3. Pieper, F. R., Schaart, G., Krimpenfort, P. J., Henderik, J. B., Moshage, H. J., van de Kemp, A., Ramaekers, F. C., Berns, A., and Bloemendal, H. (1989). Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells, J Cell Biol 108, 1009-24. 4. Raats, J. M., Pieper, F. R., Vree Egberts, W. T., Verrijp, K. N., Ramaekers, F. C., and Bloemendal, H. (1990). Assembly of amino-terminally deleted desmin in vimentin-free cells, J Cell Biol 111, 1971-85. 5. Ramaekers, F., van Niekerk, C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G., and Vooijs, P. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55.