ADAMTS4 ELISA Kit (Rat)
Référence OKEH03412
Conditionnement : 96Wells
Marque : Aviva Systems Biology
ADAMTS4 ELISA Kit (Rat) (OKEH03412)
| Datasheets/Manuals | Printable datasheet for ADAMTS4 ELISA Kit (Rat) (OKEH03412) |
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| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology ADAMTS4 ELISA Kit (Rat) (OKEH03412) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adamts4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Adamts4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adamts4 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 40.4 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Rat A disintegrin and metalloproteinase with thrombospondin motifs 4 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Adamts4 |
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| Gene Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 4 |
| Alias Symbols | a disintegrin and metallopeptidase with thrombospondin motifs 4, A disintegrin and metalloproteinase with thrombospondin motifs 4, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4, ADAM-TS 4, ADAM-TS4, ADAMTS-4, Aggrecanase, aggrecanase-1. |
| NCBI Gene Id | 66015 |
| Protein Name | A disintegrin and metalloproteinase with thrombospondin motifs 4 |
| Description of Target | Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Cleaves aggrecan at the '392-Glu-|-Ala-393' site. |
| Uniprot ID | Q9ESP7 |
| Protein Accession # | NP_076449.1 |
| Nucleotide Accession # | NM_023959.1 |
| Protein Size (# AA) | 630 |
| Molecular Weight | 68 kDa |
