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Annexin V, CF®488A Conjugate

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Référence 29005

Conditionnement : 500UL

Marque : Biotium

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Annexin V, CF®488A Conjugate

Annexin V Conjugates

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

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Click to view timelapse. Imaging of apoptosis in staurosporine-treated HeLa cells stained with 500 ng/mL CF®594 Annexin V (red, apoptotic cell surface probe) and 100 nM Oxazole Yellow (green, dead cell nuclear stain). All nuclei are stained blue with 1 uM Hoechst.

Flow cytometry analysis of untreated and staurosporine-treated Jurkat cell stained with NucView® 488 Caspase-3 Substrate and CF®647 Annexin V.

Jurkat cells were treated with staurosporine to induce apoptosis, or with DMSO as a negative control for the times indicated, then stained for 15 minutes at room temperature with NucView® 530 Caspase-3 Substrate and CF®640R Annexin V in cell culture medium prior to flow cytometry analysis on a BD LSRII flow cytometer.

Jurkat cells were treated with DMSO (negative control, blue) or 0.1 uM, 0.25 uM, or 1 uM staurosporine (red) to induce apoptosis in the presence of NucView® 530 and CF®405M Annexin V for four hours at 37°C before analysis on a BD LSR II flow cytometer. Staining with NucView® 530 and CF®405M Annexin V increase with increasing concentration of staurosporine.

Jurkat cells were treated with 1 uM staurosporine for 4 hours, then treated (green histogram) and untreated (gray histogram) cells were each stained with 2.5 ug/mL CF®488A Annexin V and analyzed by flow cytometry in the B515 (FITC) channel.

Jurkat cells were treated with 1 uM staurosporine for 4 hours, then treated (pink histogram) and untreated (gray histogram) cells were each stained with 5 uL APC Annexin V and analyzed by flow cytometry in the R660 (APC) channel.

Jurkat cells were treated with 1 uM staurosporine for 4 hours, then treated (orange histograms) and untreated (gray histograms) Jurkats were each stained with 5 uL R-PE Annexin V and analyzed by flow cytometry in the Y585 (PE) channel.

Jurkat cells were treated with 1 uM staurosporine for 4 hours, then treated (teal filled histogram) and untreated (gray histogram) Jurkats were each stained with 2.5 ug/mL CF®740 Annexin V and analyzed by flow cytometry in the R637 (APC-750) channel. Unstained cells, both treated and untreated, are shown in black.

Jurkat cells were treated with 1 uM staurosporine for 4 hours, then treated (teal filled histogram) and untreated (gray histogram) cells were each stained with 2.5 ug/mL CF®740 Annexin V and analyzed by flow cytometry in the R763 (APC-750) channel.

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Product Description

Annexin V fluorescent conjugates can be used to detect apoptotic cells. The human anticoagulant Annexin V is a 35-36 kDa calcium-dependent phospholipid-binding protein with high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the inner leaflet of the cytoplasmic membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, where it can be detected by fluorescently labeled Annexin V.

Detect apoptotic cells with fast, reliable detection of phosphatidylserine externalization
Conjugates with wide choice of CF® Dye colors, plus biotin, R-PE, APC, and other fluorescent dyes
Biotium's CF® Dyes have superior brightness, photostability, and are water-soluble
Can be used for fluorescence microscopy, live cell imaging, and flow cytometry

CF® Dye, biotin, and FITC conjugates of Annexin V are provided as 50 ug/mL solutions in 10 mM Tris, 1 mM EDTA, 30 mM NaCl, 1 mg/mL bovine serum albumin, 0.1% sodium azide, pH 7.5. R-PE and APC Annexin V conjugates are supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide at 5 uL per test.

Click to view timelapse.
Imaging of apoptosis in staurosporine-treated HeLa cells stained with CF®594 Annexin V (red, apoptotic cell surface probe) and Oxazole Yellow (green, dead cell nuclear stain). All nuclei are stained blue with Hoechst.

Detect Apoptosis

We also offer Annexin V Conjugates, Azide-Free and Lyophilized in colors ranging from UV to far-red for live cell staining, and Near-IR CF® Dye Annexin V (also lyophilized and preservative-free), compatible with small animal NIR in vivo imaging. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

Annexin V Conjugates

Product	Ex/Em	Concentration	Size	Catalog No.
CF®350 Annexin V	347/448 nm	50 ug/mL	0.5 mL	Purchase 29012
CF®405M Annexin V	408/452 nm	50 ug/mL	0.5 mL	Purchase 29009
CF®450 Annexin V	450/538 nm	50 ug/mL	0.5 mL	Purchase 29083
CF®488A Annexin V	490/515 nm	50 ug/mL	0.5 mL	Purchase 29005
CF®555 Annexin V	555/565 nm	50 ug/mL	0.5 mL	Purchase 29004
CF®568 Annexin V	562/583 nm	50 ug/mL	0.5 mL	Purchase 29010
CF®583R Annexin V	586/609 nm	50 ug/mL	0.5 mL	Purchase 29085
CF®594 Annexin V	593/614 nm	50 ug/mL	0.5 mL	Purchase 29011
CF®633 Annexin V	630/650 nm	50 ug/mL	0.5 mL	Purchase 29008
CF®640R Annexin V	642/662 nm	50 ug/mL	0.5 mL	Purchase 29014
CF®647 Annexin V	650/665 nm	50 ug/mL	0.5 mL	Purchase 29003
CF®660R Annexin V	663/682 nm	50 ug/mL	0.5 mL	Purchase 29069
CF®740 Annexin V	742/767 nm	50 ug/mL	0.5 mL	Purchase 29126
R-PE Annexin V	496, 546, 565/578 nm	5 uL/test	100 uL	Purchase 29045-100uL
R-PE Annexin V	496, 546, 565/578 nm	5 uL/test	500 uL	Purchase 29045-500uL
APC Annexin V	650/660 nm	5 uL/test	100 uL	Purchase 29057-100uL
APC Annexin V	650/660 nm	5 uL/test	500 uL	Purchase 29057-500uL
FITC Annexin V	496/516 nm	50 ug/mL	0.5 mL	Purchase 29001
Biotin Annexin V	N/A	50 ug/mL	0.5 mL	Purchase 29013
5X Annexin V Binding Buffer	N/A	5X	15 mL	Purchase 99902

CF is a registered trademark of Biotium, Inc. Alexa Fluor, and DyLight are registered trademarks of Thermo Fisher Scientific.

Technology

Cell Viability & Apoptosis

CF® Dyes

Tech Tips

Troubleshooting Tips for Fluorescence Staining

Protocol: Immunofluorescence Staining of Cells for Microscopy

Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst

Tech Tip: Fluorescence Bleed-Through and Cross-Talk

Product Attributes

Storage Conditions

Store at 2 to 8 °C, Do not freeze, Protect fluorescent conjugates from light

Size

100 uL, 500 uL

Apoptosis/viability marker

Phosphatidylserine/Annexin V

For live or fixed cells

For live/intact cells

Assay type/options

Endpoint assay, Homogeneous assay, No-wash staining, Real-time imaging, Short term staining (<24h)

Detection method/readout

Fluorescence microscopy, Live cell imaging, Flow cytometry

Format

Fluorescent dye & biotin conjugates: Supplied in 10 mM Tris pH 7.5, 1 mM EDTA, 30 mM NaCl, 1 mg/mL BSA, 0.1% sodium azide R-PE & APC conjugates: Supplied in PBS, 5 mg/mL BSA, 0.05% sodium azide

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Fixation options

Fix after staining (formaldehyde)

Product origin

Annexin V (human); recombinant, produced in E. coli, Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.