Anti-CD3, epsilon (APC/Cy5.5) Monoclonal Antibody

Référence 228083-100ug

Conditionnement : 100ug

Marque : US Biological

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228083 CD3, epsilon (APC/Cy5.5)

Clone Type
Polyclonal
Host
hamster
Source
mouse
Isotype
IgG
Grade
Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. chain of the CD3/TCR complex of mouse strains tested.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 0.3ug/10e6 cells|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC/Cy5.5 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG|Clone No: 500-A2|Host: hamster|Source: mouse|Concentration: ~0.1mg/ml |Form: Supplied as a liquid in BBS, sodium azide. Labeled with Allophycocyanin (APC/Cy5.5). |Purity: Purified|Immunogen: Immune complexes isolated from C6VL-BS thymoma cells.|Specificity: Recognizes mouse CD3.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Immune complexes isolated from C6VL-BS thymoma cells.
Form
Supplied as a liquid in BBS, sodium azide. Labeled with Allophycocyanin (APC/Cy5.5).
Purity
Purified
Specificity
Recognizes mouse CD3.
References
1. Allison JP, Havran WL, Poenie M, Kimura J, DeGraffenreid L, Ajami M, et al. Expression and function of CD3 on murine thymocytes. In: Davis MM, Kappler J, editors. The T-Cell Receptor, UCLA Symposia, 73rd Edition. New York: Alan R. Liss; 1988. p.33-45. (Immunogen, FC, IP, Activ) 2.Swallow MM, Wallin JJ, Sha WC. B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNF. Immunity. 1999;11:423-32. (Activ) 3. Hörnquist CE, Lu X, Rogers-Fani PM, Rudolph U, Shappell S, Birnbaumer L, et al. Gi2-deficient mice with colitis exhibit a local increase in memory CD4+ T cells and proinflammatory Th1-type cytokines. J Immunol. 1997;158:1068-77. (IHC-FS) 4. Portoles P, Rojo J, Golby A, Bonneville M, Gromkowski S, Greenbaum L, et al. Monoclonal antibodies to murine CD3 define distinct epitopes one of which may interact with CD4 during T cell activation. J Immunol. 1989;142:4169-75. (Activ)