Anti-Human IFNγ
Référence I-141-25
Conditionnement : 25ug
Marque : Leinco Technologies
AntiHuman IFNγ
AntiHuman IFNγ
Product No.: I141
Target IFNγ Product Type Polyclonal Antibody Alternate Names Interferon Gamma, Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI Applications ICC , N , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human IFNγ (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at 20°C to 70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 28°C. For longterm storage of the reconstituted antibody, aseptically aliquot into working volumes and store at 20°C to 70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830535 Applications and Recommended Usage? Quality Tested by Leinco Western Blot: Suitable for use at concentration of 0.1 µg/mL. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human IFNγ bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IFNγ (at 5 ng/ml) is 0.10.3 µg/ml. Immunocytochemistry: Suitable at concentration of 515 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat AntiHuman InterferonGamma (IFNγ) recognizes Human IFNγ. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interferongamma (IFNγ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigenspecific immunity develops.2 IFNγ has antiviral, immunoregulatory, and antitumour properties.3 PubMed NCBI Gene Bank ID UniProt.org Research Area Other Molecules References & Citations1. Goeddel, DV. et al. (1982) Nature 298: 859 2. Wilson, CB. et al. (2007) Adv. Immunol. 96: 41 3. Hume, DA. et al. (2004) J Leukoc Biol. 75: 163 |