R1001-1mg | ARCA EGFP mRNA Clinisciences

EGFP (enhanced green fluorescent protein) mRNA can express an enhanced green fluorescence once has been transfected into cells, which is a direct-detection reporter mRNA, used as control to study transfection and expression in mammalian cells by various fluorescence-based assays. You can observe the fluorescence at 509 nm.

Our product had already been modified with ARCA with a high efficient co-transcriptional capping method, which means most of mRNA has a Cap 0 structure. It is a more mature mRNA than mRNA with no capping and maintain a more stable state which lead to higher expressing efficiency in transfected mammalian cells. ARCA obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with mCAP.

A upgraded version of ARCA EGFP mRNA is available, please check out R1007 ARCA EGFP MRNA (5-moUTP) for more details.

1. Yan Liang, Jingge Zhang, et al. "Biomimetic Mineralized CRISPR/Cas RNA Nanoparticles for Efficient Tumor-Specific Multiplex Gene Editing." ACS Nano. 2023 Aug 8;17(15):15025-15043. PMID: 37481734
2. Hao Chen, Lina Guo, et al. "A General and Efficient Strategy for Gene Delivery Based on Tea Polyphenols Intercalation and Self‐Polymerization." Adv Sci (Weinh). 2023 Aug;10(24):e2302620. PMID: 37349886
3. Qiming Yin, Xiang Song, et al. "Incorporation of glycyrrhizic acid and polyene phosphatidylcholine in lipid nanoparticles ameliorates acute liver injury via delivering p65 siRNA." Nanomedicine. 2022 Dec 27;48:102649. PMID: 36584740

Identity & Purity

Agarose Gel Mobility: Pass.
Concentration ± 6%: Pass.

QC
MSDS (Material Safety Data Sheet)
Datasheet

Related Biological Data

mRNA Length	996 nucleotides
Concentration	1 mg/mL
Buffer	1 mM Sodium Citrate, pH 6.4	Storage	-40°C or below
General tips	Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent.
Shipping Condition	Evaluation sample solution : ship with dry ice.

Cell line	HEK293T
Tissue culture plate	48-well tissue culture plate
The plating cell volume	50,000
Cell density before transfection	cell fusion degree 50%-60%
Transfection conditions	After culturing for 17 hours, transfection (1μg mRNA+2μL lipofectamine 2000) in serum- and antibiotic-free medium, 4 hours later, change to complete medium to return to normal growth environment and perform fluorescence photography after 24 hours.
RNA amount and transfection reagent	1μg mRNA and 2μL lipofectamine 2000 are prepared according to the instructions
Transfection efficiency	above 90% transfection efficiency

ARCA EGFP mRNA

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Identity & Purity

Related Biological Data

Description

Protocol

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