Blue Gel Loading Buffer with DNA Stain

Référence orb533360-5x1.8ml

Conditionnement : 5x1.8ml

Marque : Biorbyt


Blue Gel Loading Buffer with DNA Stain

SKU: orb533360

Description

Biorbyt Gel Loading Buffers with DNA Stain are formulated to facilitate loading of DNA samples into the wells of agarose and polyacrylamide gels. The loading buffers contain SYBR Green DNA Stain a fluorescent DNA intercalator dye specially developed for DNA analysis applications. High quantum yield and excellent stability makes SYBR Green the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes Ethidium Bromide. The buffers contain tracking dyes as indicator for DNA fragment migration. In addition, they contain glycerol to add density and EDTA to inhibit nuclease activities.

Images & Validation

Application Notes
Blue Gel Loading buffer with DNA Stain is recommended for analyzing DNA fragments ≥ 500 bp. Add one part of Gel Loading Buffer to 5 parts of the DNA sample as shown in the table below. Spin down the tubes and vortex gently to achieve homogeneity. DNA sample: 5 µl 10 µl 20 µl 50 µl; 6x Gel Loading Buffer: 1 µl 2 µl 4 µl 10 µl.

Key Properties

Storage & Handling

Storagestore at -20 °C
Form/AppearanceLiquid
Concentration6x conc
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Loading buffer for agarose or polyacrylamide gels with green-fluorescent DNA stain

Protocol Information