Bovine Interleukin 1,IL-1 ELISA Kit
Référence CSB-E12019B-10x96T
Conditionnement : 10x96T
Marque : Cusabio
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Interleukin 1,IL-1
Associated names
IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
Abbreviation
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
250 pg/mL-4000 pg/mL
Sensitivity
250 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-97 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 90-102 | |||||
Recovery
| The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 89 | 83-96 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Bovine IL1A ELISA Kit was designed for the quantitative measurement of Bovine IL1A protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 250 pg/mL-4000 pg/mL and the sensitivity is 250 pg/mL.
Citations
- F Ma,Animals,2024
- P Jiao,Canadian Journal of Animal Science ja,2021
- Zhang G.et al,Res Vet Sci,2017
- Zhang G.et al,Research in Veterinary Science,2016
- Mustafa Kabu. et al,/,2016
- Dervishi E.et al,J Anim Sci Biotechnol.,2016
- ALBAYRAK H,.et al,/,2016
- Lv E. et al,Free Radic Res.,2015
- Dervishi E.et al,J Anim Sci Technol.,2015
- Iqbal S et al,Innate Immun,2013
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Gene References into Functions
- These findings suggest that the elevated levels of IL-1alpha found in the osteoarthritis environment heighten fibroblast-like synoviocytes sensitivity to fluid shear by altering both intercellular communication and individual cell sensitivity, which could affect downstream functions and contribute to progression of the disease state
- Protein kinase R plays a pivotal role in oncostatin M and interleukin-1 signalling in bovine articular cartilage chondrocytes.
- Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period.
- Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase.
- non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties
- These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells.
Subcellular Location
Cytoplasm. Secreted.
Protein Families
IL-1 family
Database Links
KEGG:
STRING:
UniGene: