CA2 siRNA (Mouse)
Référence MBS827046-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
CA2 siRNA – Carbonic Anhydrase 2 (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827046MBS827046 is an siRNA targeting CA2. Also known as CA2 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit CA2 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
CA2, siRNA
Full Product Name
CA2 siRNA (Mouse)
Product Gene Name
CA2 sirna
Product Synonym Names
CAR2; Carbonic anhydrase 2; Carbonate dehydratase II; Carbonic anhydrase II; CA-II
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
29,033 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CA2 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of CA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for CA2. It may not necessarily be applicable to this product.
NCBI Official Full Name
carbonic anhydrase 2
NCBI Official Synonym Full Names
carbonic anhydrase 2
NCBI Official Symbol
Car2
NCBI Official Synonym Symbols
Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712
UniProt Protein Name
Carbonic anhydrase 2
UniProt Synonym Protein Names
Carbonate dehydratase II; Carbonic anhydrase II; CA-II
Protein Family
Beta-carotene hydroxylase
UniProt Gene Name
Ca2
UniProt Synonym Gene Names
Car2; CA-II
UniProt Entry Name
CAH2_MOUSE
NCBI Protein Information
carbonic anhydrase 2
UniProt Comments for CA2
CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.
Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase
Cellular Component: extracellular space; microvillus; membrane; apical part of cell; basolateral plasma membrane; cell; axon; cytoplasm; plasma membrane; cytosol; myelin sheath
Molecular Function: carbonate dehydratase activity; zinc ion binding; metal ion binding; lyase activity
Biological Process: secretion; positive regulation of osteoclast differentiation; carbon dioxide transport; regulation of intracellular pH; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; positive regulation of bone resorption; regulation of pH; morphogenesis of an epithelium; one-carbon compound metabolic process
Show more Protein type: Energy Metabolism - nitrogen; EC 4.2.1.1; Lyase
Cellular Component: extracellular space; microvillus; membrane; apical part of cell; basolateral plasma membrane; cell; axon; cytoplasm; plasma membrane; cytosol; myelin sheath
Molecular Function: carbonate dehydratase activity; zinc ion binding; metal ion binding; lyase activity
Biological Process: secretion; positive regulation of osteoclast differentiation; carbon dioxide transport; regulation of intracellular pH; positive regulation of cellular pH reduction; positive regulation of synaptic transmission, GABAergic; positive regulation of bone resorption; regulation of pH; morphogenesis of an epithelium; one-carbon compound metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with CA2 sirna
| Reference Product | PubMed Publications |
|---|---|
| Slc4a4 sirna | >4 publications with CA2 and Slc4a4 |
| Slc26a4 sirna | >3 publications with CA2 and Slc26a4 |
| Cpa2 sirna | >2 publications with CA2 and Cpa2 |
| Slc26a6 sirna | >1 publications with CA2 and Slc26a6 |
| Slc9a1 sirna | >1 publications with CA2 and Slc9a1 |
| Calb1 sirna | >1 publications with CA2 and Calb1 |
| Slc4a1 sirna | >1 publications with CA2 and Slc4a1 |
Pathways associated with CA2 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Bile Secretion Pathway antibodies | Bile Secretion Pathway Diagram |
| Bile Secretion Pathway antibodies | Bile Secretion Pathway Diagram |
| Collecting Duct Acid Secretion Pathway antibodies | Collecting Duct Acid Secretion Pathway Diagram |
| Collecting Duct Acid Secretion Pathway antibodies | Collecting Duct Acid Secretion Pathway Diagram |
| Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway antibodies | Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway Diagram |
| Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway antibodies | Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway Diagram |
| Gastric Acid Secretion Pathway antibodies | Gastric Acid Secretion Pathway Diagram |
| Gastric Acid Secretion Pathway antibodies | Gastric Acid Secretion Pathway Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
| Nitrogen Metabolism Pathway antibodies | Nitrogen Metabolism Pathway Diagram |
Diseases associated with CA2 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Fibrosis Antibodies | >3 publications with CA2 and Fibrosis |
| Cardiovascular Diseases Antibodies | >3 publications with CA2 and Cardiovascular Diseases |
| Inflammation Antibodies | >3 publications with CA2 and Inflammation |
| Necrosis Antibodies | >3 publications with CA2 and Necrosis |
| Nervous System Diseases Antibodies | >3 publications with CA2 and Nervous System Diseases |
| Disease Models, Animal Antibodies | >2 publications with CA2 and Disease Models, Animal |
| Fatty Liver Antibodies | >1 publications with CA2 and Fatty Liver |
| Hypertrophy Antibodies | >1 publications with CA2 and Hypertrophy |
| Edema Antibodies | >1 publications with CA2 and Edema |
| Kidney Diseases Antibodies | >1 publications with CA2 and Kidney Diseases |
Organs/Tissues associated with CA2 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Kidney Antibodies | >8 publications with CA2 and Kidney |
| Brain Antibodies | >6 publications with CA2 and Brain |
| Eye Antibodies | >5 publications with CA2 and Eye |
| Liver Antibodies | >4 publications with CA2 and Liver |
| Uterus Antibodies | >4 publications with CA2 and Uterus |
| Muscle Antibodies | >4 publications with CA2 and Muscle |
| Intestine Antibodies | >3 publications with CA2 and Intestine |
| Blood Antibodies | >3 publications with CA2 and Blood |
| Bone Antibodies | >3 publications with CA2 and Bone |
| Lung Antibodies | >3 publications with CA2 and Lung |