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> CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (FITC)

CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (FITC)

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Référence C2433-12D-100ug

Conditionnement : 100ug

Marque : US Biological


C2433-12D CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (FITC)

Clone Type
Monoclonal
Host
hamster
Source
mouse
Isotype
IgG
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo Rt Rd
Shipping Temp
Blue Ice
Storage Temp
-20°C

Fusion Partners: Spleen cells from immunized Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.

Recommended Negative Controls: Hamster IgG Negative Control: FITC

Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG|Clone No: Eat 2|Host: hamster|Source: mouse|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC). |Purity: Purified by Protein G affinity chromatography.|Immunogen: 38C13, murine B cell line.|Specificity: Recognizes mouse and rat CD81, a 26kD cell surface glycoprotein that is also known as TAPA-1.In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. 3H1702 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. 3H1702 requires the presence of both extracellular loops of TAPA-1 for binding. Species Crossreactivity: Mouse, rat ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
38C13, murine B cell line.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse and rat CD81, a 26kD cell surface glycoprotein that is also known as TAPA-1.In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. 3H1702 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation. 3H1702 requires the presence of both extracellular loops of TAPA-1 for binding. Species Crossreactivity: Mouse, rat
References
1. Clark, K. et al. (2001). PGRL is a major CD81 - associated protein on lymphocytes and distingushes a new family of cell surface proteins. J. Immunol. 167: 5115 - 5121.