CF®488A TUNEL Assay Apoptosis Detection Kit
Référence 30063
Conditionnement : 1kit
Marque : Biotium
TUNEL Assay Apoptosis Detection Kit
The CF® dye TUNEL Assay Apoptosis Detection Kit label apoptotic cells in fixed tissue sections or cellular samples for fluorescence microscopy or flow cytometry.
Apoptotic cells in a section of involuting mouse mammary gland stained with CF®594 TUNEL Assay (red). Nuclei are counterstained blue with DAPI.
Product Description
These kits use enzymatic labeling of DNA double-strand breaks for fluorescence staining of apoptotic cells in fixed samples or tissue sections by TUNEL.
Features
- Direct labeling of DNA ds breaks with fluorescent dUTP
- Bright & photostable next-generation CF® dyes
- For fixed cells or tissue sections
- Detect by fluorescence microscopy or flow cytometry
Kit Components
- Equilibration Buffer
- CF® Dye TUNEL Reaction Buffer
- TdT Enzyme (calf thymus; recombinant, produced in E. coli)
Note: Kits include reagents for 50 reactions using 50 uL per reaction.
In TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling), TdT enzyme catalyzes the addition of labeled dUTP to the 3'- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF® dye TUNEL Assay Apoptosis Detection Kits employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry. Learn more about Biotium's next-generation CF® Dyes.
See our full selection of Cell Viability & Apoptosis Kits.
CF® Dye TUNEL Assay Apoptosis Detection Kits
| Dye | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®488A | 490/515 nm | 50 reactions | 30063 |
| CF®594 | 593/614 nm | 50 reactions | 30064 |
| CF®640R | 642/662 nm | 50 reactions | 30074 |
Technology
Tech Tips
Powered by Bioz