Cholesteryl acetate [604-35-3]

Cholesteryl acetate (Cholesterol 3-acetate) is a cholesterol ester that is exported from Saccharomyces cerevisiae via a Pry1-dependent mechanism. Cholesteryl acetate binds to the CAP superfamily protein Pry1 via interactions dependent on Pry1’s caveolin-binding motif^[1][2].

Cholesteryl acetate (50 pmol; 1 h) requires the caveolin-binding motif of purified Saccharomyces cerevisiae Pry1 for selective binding, as mutations in this motif eliminate cholesteryl acetate competition with cholesterol for binding, while other mutations do not affect this interaction^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

• [1]. Z Huang, et al. Solubility of cholesterol and its esters in supercritical carbon dioxide with and without cosolvents. The Journal of Supercritical Fluids. Volume 30, Issue 1. 2004. Pages 25-39.

  [2]. Choudhary V, et al. The caveolin-binding motif of the pathogen-related yeast protein Pry1, a member of the CAP protein superfamily, is required for in vivo export of cholesteryl acetate. J Lipid Res. 2014;55(5):883-894.  [Content Brief]