Collagen I (COL1A1) rabbit polyclonal antibody, Purified
Size:
100 ul
Conjugation:
Unconjugated
View other "Collagen I or NP_000079" antibodies
Specifications
Product Data
Application
ELISA, IF, IHC, R
Recommended Dilution
ELISA: > 1/200. RIA. Immunofluorescence: 1/80. Immunohistochemistry on Paraffin Sections: 1/200-1/600 for 60 min at RT or 2-8°C over night. Positive Control: Tuna Fish skin. Pretreatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.
Reactivity
Bonito, Fish, Tuna
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
Purified Collagen type I from Tuna Fish skin
Specificity
This antibody is specific for Fish Collagen type I (Tuna Fish).
Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms
COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
