Coronavirus, Nucleoprotein (NL63), Recombinant

Severe acute respiratory syndrome (SARS) is a lower respiratory tract illness that was first reported in patients from the Guandong Province of China in November 2002. The causative agent, which was previously unknown, was isolated in 2003 and named as SARS Coronavirus (SARS-CoV). The SARS Coronavirus is an enveloped, single-stranded, positive RNA virus of the family Coronoviridae (NCBI) The virus is
thought to have a zoonotic origin, with the horseshoe bat being the primary natural reservoir, but this has not been confirmed. Mammals, including the palm civet, may act as intermediate hosts. In 2003, the SARS Coronavirus spread rapidly and affected over 8,000 people in 26
countries. The rapid spread of SARS-CoV is thought to be due to person-to-person transmission of the virus via aerosol droplets or close contact with infected individuals. Since the end of the SARS-CoV epidemic, cases of SARS infection have only occurred in laboratory workers that have been accidently infected (WHO). The symptoms of SARS infection are like influenza and include fever, malaise, muscle pain, headache, diarrhea and shivering. Clinical symptoms may also include coughing and shortness of breath. Respiratory distress may rapidly develop in some patients, resulting in death. SARS disease has a high rate of mortality and resulted in
774 deaths during the first epidemic in 2003. Currently, no licensed vaccine is available for the prevention of SARS infection. SARS
infection continues to be of global health concern due to the rapid spread of the virus, the high mortality rate and the fears of a future SARS outbreak

Recombinant protein corresponding to Coronavirus (CoV Nucleoprotein NL63), expressed in E. coli.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.