ENaC Beta Antibody
Référence OASE00461
Conditionnement : 100ug
Marque : Aviva Systems Biology
ENaC Beta Antibody (OASE00461)
| Datasheets/Manuals | Printable datasheet for ENaC Beta Antibody (OASE00461) |
|---|---|
| COA Datasheet | 1 µg/ml of SPC-404 was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Predicted Species Reactivity | Hamster|Human|Mouse|Rat|Xenopus|Xenopus laevis |
|---|---|
| Product Format | Liquid PBS with 0.09% sodium azide and 50% glycerol |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | ICC,IF,IHC,IP,WB |
| Additional Information | Certificate of Analysis: 1 ug/mL of OASE00461 was sufficient for the detection of beta-ENaC in 20 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. Cellular Localization: Apical cell membrane. |
| Reconstitution and Storage | -20°C |
| Immunogen | Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2) |
| Purification | Protein A Purified |
| Concentration | 1 mg/ml |
| Specificity | Detects ~87kDa. |
| Dilution | WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user. |
| Application Info | WB: 1:1000 IHC: 1:100. Optimal dilutions for assays should always be determined by the user. |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Reference | 1. Kakizoe Y., et al. (2009) J Hpyertens. 27(8): 1679-1689. 2. Gu Y. (2008) J Cell Physiol. 216(2):453-457. 3. Bruns J.B. (2003) Am J Physiol Renal Physiol. 285(4): F600-F609. 4. Mauro T., et al. (2002) J Invest Dermatol. 118(4): 589-594. 5. Elias N., et al. (2007) Am J Physiol Lung Cell Mol Physiol. 293(3): L537-45. 6. Dyka F.M., May C.A. and Enz R. (2005) J Neurochem. 94(1): 120-128. |
|---|---|
| Gene Symbol | Scnn1b |
| Gene Full Name | sodium channel epithelial 1 subunit beta |
| Alias Symbols | amiloride-sensitive sodium channel subunit beta, beta-ENaC, beta-NaCH, epithelial Na(+) channel subunit beta, nonvoltage-gated sodium channel 1 subunit beta, RNENACB, SCNEB, sodium channel epithelial 1 beta subunit, sodium channel, non-voltage-gated 1, beta subunit, sodium channel, nonvoltage-gated, type I, beta. |
| NCBI Gene Id | 24767 |
| Protein Name | Amiloride-sensitive sodium channel subunit beta|amiloride-sensitive sodium channel subunit beta [Rattus norvegicus] |
| Description of Target | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. |
| Uniprot ID | P37090 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_036780 |
