EXPB-2 Polyclonal Antibody
Référence bs-0243R
Conditionnement : 100uL
Marque : Bioss
Paraformaldehyde-fixed, paraffin embedded Tomato leaf; Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with EXPB-2 Polyclonal Antibody, Unconjugated (bs-0243R) at 1:400 overnight at 4\u2103, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Applications
Reactivity
| Overview | |
| Catalog # | bs-0243R |
| Product Name | EXPB-2 Polyclonal Antibody |
| Applications | WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
| Reactivity | Plant |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Triticum aestivum EXPB-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Swiss Prot | Q6QN86 |
| Synonyms | beta-expansin 2; expansin EXPB5; Q6QN86_WHEAT. |
| Background | beta-expansin (EXBP2) may cause loosening and extension of plant cell walls by disrupting noncovalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found Subcellular Location at Cell-wall bound. Belongs to the expansin family. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |