FGF-7/KGF Protein, Mouse, Recombinant (His)

Référence NB-64-59264-5ug

Conditionnement : 5ug

Marque : Neo Biotech


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FGF-7/KGF Protein, Mouse, Recombinant (His)

(Synonyms: Kgf, fibroblast growth factor 7) Copy Product Info

Synonyms: Kgf, fibroblast growth factor 7

Catalog No. TMPY-04206 Copy Product Info
FGF-7/KGF Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 21 kDa and the accession number is P36363.
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 90 % as determined by SDS-PAGE
Appearance:Solution
Color:Transparent
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Product Information

Bioactivity
Bioactivity
1.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGFR2b. The ED50 for this effect is typically 20-100 ng/mL. 2.Mouse Bladder Organoids after 14 days in culture.25 ng/mL of Recombinant Mouse KGF/FGF-7 Protein (50394-M07E) was used for 2 weeks in BME submerged in mouse bladder media.Data provided by Dr. Yin at Washington University, St. Louis, MO.
Description
FGF-7/KGF Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 21 kDa and the accession number is P36363.
Species
Mouse
Expression System
E. coli
TagN-His
Accession NumberP36363
ConstructionA DNA sequence encoding the mouse FGF7 (NP_032034.1) (Cys32-Thr194) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity
> 90 % as determined by SDS-PAGE
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 10 mM Na2HPO4, 1. 8 mM KH2PO4, 2. 7 mM KCl, 140 mM NaCl, 15% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsKgf, fibroblast growth factor 7
Research Background
Fibroblast growth factor 7 (FGF7) is a member of the fibroblast growth factor (FGF) family of proteins. FGF7 plays an important role in regulating the proliferation, migration, and differentiation of cells. FGF7 is of stromal origin and produces a paracrine effect on epithelial cells. FGF7 is a mesenchyme-specific heparin-binding growth factor that binds FGF receptor 2 (FGFR2) to regulate numerous cellular and physiological processes. FGF7/FGFR2 promotes invasion and migration in human gastric cancer. FGF7 is specifically utilized as a paracrine factor during the process of differentiation of the epidermal layers in the regenerating scales and in particular for beta-cells differentiation. FGF7 over expression is associated with advanced clinical features in patients with upper tract and bladder urothelial carcinoma, justifying its potential prognostic value for urothelial carcinoma.
Chemical Properties
Molecular Weight21 kDa (predicted); 25 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: FGF-7/KGF Protein, Mouse, Recombinant (His) chemical structure | FGF-7/KGF Protein, Mouse, Recombinant (His) molecular weight