Fibronectin (FN) Recombinant, Human

Recombinant protein corresponding to aa2206-2337 (P02751) with a N-Terminal His-Tag from human Fibronectin expressed in E. coli

Predicted Molecular Weight:
16.1kD

Predicted Isoelectric Point:
5.3

Subcellular Location:
Secreted, extracellular space, extracellular matrix

Amino Acid Sequence:
ALSQTT ISWAPFQDTS EYIISCHPVG TDEEPLQFRV PGTSTSATLT GLTRGATYNV IVEALKDQQR HKVREEVVTV GNSVNEGLNQ PTDDSCFDPY TVSHYAVGDE WERMSESGFK LLCQCLGFGS GHFRCD

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE.
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Included Protein Molecular Weight Marker:
168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed.
MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD
Double intensity: 10kD, 18kD, 26kD
Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.