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Harringtonine [26833-85-2]

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Référence NB-64-44042-1mg

Conditionnement : 1mg

Marque : Neo Biotech

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Harringtonine

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Synonyms:

Catalog No. T8286 Copy Product Info

Purity: 99.88%

Harringtonine is a naturally occurring alkaloid that inhibits DNA synthesis and protein synthesis and strongly kills S-phase cells. Harringtonine has antitumor and antiviral activities.

Cas No. 26833-85-2

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.88%

Color:White to Brown

Product Introduction

Bioactivity

Description	Harringtonine is a naturally occurring alkaloid that inhibits DNA synthesis and protein synthesis and strongly kills S-phase cells. Harringtonine has antitumor and antiviral activities.
Targets & IC50	HeLa cells:4 μM, HSV1:0.1081-0.1584 μM, CHIKV:0.24 μM (EC50), HL-60 cells:0.047 μM, BHK-21 cells:0.24 μM (EC50)
In vitro	Harringtonine inhibits the elongation phase of translation by preventing substrate binding to the acceptor site on the 60-S ribosome subunit, blocking aminoacyl-tRNA binding and peptide bond formation [1]. It displays potent inhibition of Chikungunya virus infection (EC50: 0.24 μM) and could inhibit other alphaviruses [2]. Additionally, Harringtonine inhibits the growth of human myeloid leukemia cells in vitro at low concentrations [3].
In vivo	METHODS: To study in vivo activity, Harringtonine (0.5-4 mg/mouse) was injected retroorbital into C57BL/6 mice. RESULTS: Within 5 min, Harringtonine completely depleted multimers in all organs except testis and brain, and it took up to 30 min to significantly reduce the number of multimers due to the blood-brain and blood-testis barriers. [3]

Chemical Properties

Molecular Weight	531.59
Formula	C₂₈H₃₇NO₉
Cas No.	26833-85-2
Smiles	COC(=O)C[C@](O)(CCC(C)(C)O)C(=O)O[C@H]1[C@H]2c3cc4OCOc4cc3CCN3CCC[C@]23C=C1OC
Relative Density.	1.35 g/cm3

Storage & Solubility Information

Storage

Keep away from direct sunlight,Store under nitrogen,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (103.46 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.76 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8811 mL	9.4057 mL	18.8115 mL	94.0575 mL
5 mM	0.3762 mL	1.8811 mL	3.7623 mL	18.8115 mL
10 mM	0.1881 mL	0.9406 mL	1.8811 mL	9.4057 mL
20 mM	0.0941 mL	0.4703 mL	0.9406 mL	4.7029 mL
50 mM	0.0376 mL	0.1881 mL	0.3762 mL	1.8811 mL
100 mM	0.0188 mL	0.0941 mL	0.1881 mL	0.9406 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Keywords

InhibitorinhibitInfluenzaVirusInfluenza VirusHarringtonine

Related Tags: Harringtonine chemical structure | Harringtonine in vivo | Harringtonine in vitro | Harringtonine formula | Harringtonine molecular weight