Human IgG gamma Antibody Alkaline Phosphatase
Référence orb347239-1mg
Conditionnement : 1mg
Marque : Biorbyt
Description
Research Area
Signal Transduction
Images & Validation
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| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:4,000 - 1:16,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Human IgG gamma heavy chain |
| Purity | Human IgG (gamma chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), Human IgG, Human serum and anti-Goat Serum. Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes. |
| Conjugation | AP |
Storage & Handling
−| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−goat anti-Human IgG (gamma chain) alkaline phosphatase conjugated Antibody, goat anti-Human IgG Antibody alk phos conjugation
−
Human IgG (gamma chain) Antibody Alkaline Phosphatase Conjugated [orb347302]
DOT, ELISA, IHC, WB
Human
Rabbit
Polyclonal
AP
1 mg
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)