IgG3 (Biotin)
Référence I1904-85G-1mg
Conditionnement : 1mg
Marque : US Biological
I1904-85G IgG3 (Biotin)
Clone Type
PolyclonalHost
rabbitSource
mouseIsotype
IgGGrade
Affinity PurifiedApplications
E IHC WBCrossreactivity
MoShipping Temp
Blue IceStorage Temp
4°C (-20°C Glycerol)
Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy. Other applications not tested.
Recommended Dilution:
ELISA: 1:8000 to 1:32,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:2000
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml (UV280)|Form: Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol. |Purity: Purified by immunoaffinity chromatography.|Immunogen: Mouse IgG3 heavy chain.|Specificity: Recognizes mouse IgG3. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other Mouse heavy chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Mouse IgG3 heavy chain.
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse IgG3. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Less than 1% crossreactivity was observed against other Mouse heavy chain isotypes.