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> Insulin (INS) Mouse Monoclonal Antibody [Clone ID: E2-E3+2D11-H5 (INS04+ INS05)]

Insulin (INS) Mouse Monoclonal Antibody [Clone ID: E2-E3+2D11-H5 (INS04+ INS05)]

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Référence AM33363PU-S

Conditionnement : 100ug


Insulin / IRDN (beta-Cell & Insulinoma Marker) Mouse Monoclonal Antibody [Clone ID: E2-E3+2D11-H5; same as INS04+INS05]

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SKU
AM33363PU-S
Insulin / IRDN (beta-Cell & Insulinoma Marker) mouse monoclonal antibody, clone E2-E3+2D11-H5; same as INS04+INS05, Purified
Size:
100 ug 20 ug
Conjugation:
Unconjugated
 

View other "INS or NP_000198.1" antibodies

Specifications
Product Data
Clone Name E2-E3+2D11-H5; same as INS04+INS05
Application FC, IF, IHC, IP, WB
Recommended Dilution ELISA: Use Antibody without BSA for Coating.
Flow Cytometry: 0.5-1 μg/106 cells.
Immunofluorescence: 1-2 μg/ml.
Western Blot: 0.5-1 μg/ml.
Immunoprecipitation: 1-2 μg/500 μg protein lysate.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.1-0.2 μg/ml for 30 minutes at RT.
No special pretreatment is required for staining of formalin/paraffin tissues.
Positive Control: Pancreas.
Reactivity Bovine, Cow, Human, Mouse, Pig, Porcine, Rabbit, Rat
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Purified pig insulin, conjugated to KLH (E2-E3 & 2D11-H5)
Specificity Cellular Localization: Secreted
Buffer 200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation Unconjugated
Storage Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Predicted Protein Size 6kDa
Gene Name insulin
Database Link
Background Recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker.
Synonyms IDDM2, ILPR, IRDN, MODY10, Proinsulin
Reference Data
Product Manuals
FAQs
  • Antibody and Western Standard
SDS

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