Interferon alpha 2b, Recombinant, Human (Interferon Alpha 2b, IFN2b, IFNA, IFNbeta, IFN)

All known subtypes of IFN-alpha show the same antiviral antiparasitic, antiproliferative activities. Human IFN-alpha is also a potent antiviral substance in murine, porcine, and bovine cell systems. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino- terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. IFN-alpha and IFN-beta are thought to bind to the same receptor. Signal transduction mechanisms elicited after binding of IFN-alpha to its receptors involves tyrosine phosphorylation of various non-receptor tyrosine kinases belonging to the Janus kinases.

Recombinant Interferon-alpha 2b is a disulfide-linked monomeric protein consisting of 166 amino acid residues. Interefron alpha 2b migrates as an ~19kD protein under non-reducing conditions and reducing conditions in SDS-PAGE.

Optimized DNA sequence encoding human Interferon-alpha mature chain was expressed in E. coli. Uniprot/Accession: P01563

Biological Activity:
The ED50 was determined by viral resistance assay and was found to be ≥5x10e8 IU/mg.

Amino Acid Sequence:
MALTFYLLVALVVLSYKSFSSLGCDLQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.