Kinetic HDAC Assay Developer Kit
Référence 82936
Conditionnement : 1kit
Marque : BPS Bioscience
Kinetic HDAC Assay Developer Kit
Catalog #
Description
The Kinetic HDAC Assay Developer Kit is an essential reagent kit for signal generation in Kinetic HDAC assays performed with any of BPS Bioscience’s HDAC fluorometric substrates. The developer sensitizes the deacetylated substrate so that subsequent treatment with the HDAC assay developer produces a fluorophore.
Format
| Catalog # | Name | Amount | Storage |
| 53029 | 10x Kinetic HDAC Assay Developer Solution | 1 ml | -80°C |
| 82937 | Kinetic HDAC Assay Developer Dilution Solution | 10 ml | -80°C |
References
Ito K., et al., 2002Proc. Natl. Acad. Sci. USA 99: 8921
Heltweg B. and Jung M., 2002 Anal. Biochem. 302: 175
Milutinovic S., et al.,2002 J. Biol. Chem. 277: 20974
Stability
Store Kinetic HDAC Assay Developer Solution in aliquots, at -80°C for up to 6 months. Kinetic HDAC Assay Developer Solution is stable for at least 3 freeze-thaw cycles, but care should be taken that both freezing and thawing steps are done quickly (e.g. liquid N2 or dry ice/EtOH for freezing, rapid finger-rubbing for thawing). Do not store diluted solutions.
Instructions for Use
Dilute 10x Kinetic HDAC Assay Developer Solution (#53029) 10-fold with Kinetic HDAC Assay Developer Dilution Solution (#82937). Add 50 µl of 1x solution per each 50 µl of HDAC reaction (1:1, for a total volume 100 µl).
Applications
• Kinetic HDAC1 Assay Kit
• Kinetic HDAC2 Assay Kit
• Kinetic HDAC3 Assay Kit