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> Measles Virus HA / CD150 Ligand Binding Assay Kit

Measles Virus HA / CD150 Ligand Binding Assay Kit

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Référence MV-HA-CD150-2

Conditionnement : 2PlateKit

Marque : RayBiotech


Measles Virus HA / CD150 Ligand Binding Assay Kit

The Measles Virus (MV) HA / CD150 Ligand Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between MV Hemagglutinin and its receptor CD150 in the presence of potential inhibitors within 4 hours.

Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time 6-8 weeks
Species Human, Measles virus
Quantitative/Semi-Quantitative Semi-Quantitative
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Research Area Receptor-Ligand Binding, Infectious Disease, Measles
Shipping Type Dry ice (additional shipping fee)
Storage -80°C

Kit Components

  • Pre-coated 96-well microplate
  • Wash Buffer
  • Assay Diluent
  • Ligand Protein
  • Detection Antibody
  • HRP Conjugated Anti-IgG
  • TMB One-Step Substrate Reagent
  • Stop Solution

Additional Materials Required

  • Microplate reader capable of measuring absorbance at 450 nm
  • Shaker
  • Pipettes capable of accurately delivering 2 µL to 1 mL volumes
  • Pipettes capable of delivering 1-25 mL volumes for reagent preparation
  • 100 mL and 1 liter graduated cylinders
  • Distilled or deionized water
  • Tubes to prepare sample dilutions

Assay Procedure Summary

  1. Prepare all reagents and test reagent samples as instructed.
  2. Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
  3. Add 100 µL the prepared detection antibody solution to each well. Incubate 1 hour.
  4. Add 100 µL the prepared 1X HRP conjugated Anti-IgG solution to each well. Incubate 1 hour.
  5. Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
  6. Add 50 µL stop solution directly to each well. Read at 450 nm immediately.

Storage/Stability

The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.