The Monkeypox Virus (MPXV) H3L Protein Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG antibody against MPXV Envelope Protein H3L in human serum or plasma. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.
Monkeypox Virus H3L Protein Human IgG ELISA Kit
Monkeypox Virus H3L Protein Human IgG ELISA Kit
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Monkeypox Virus |
| Compatible Sample Types | Plasma, Serum |
| Gene Symbols | IGG |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Mpox |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -80°C |
Application Notes
Kit Components
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µL positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
