Nanobacteria Removal Medium (M199)

Nanobacteria and their decomposition complexes are the common contaminant in cell cultures that co-exists with cells. Antibiotics are usually ineffective. Nanobacteria grows competitively with cells, which is unfavorable to cell growth, and in severe cases causes cell death. At present, many cells are contaminated with nanobacteria, which has a great impact on cell culture and subsequent experiments. The common characteristics of cells contaminated by nanobacteria are as follows: (1) The medium is not turbid, but when the cells are observed under a microscope, there are many "small black spots" around the cells or in the culture medium. With the extension of culture time, the "small black spots" gradually increase, and they cannot be removed by changing the culture medium or washing the cells; (2) the cells contaminated by the "small black spots" consume fast nutrients and require frequent replacement of the culture medium; (3) Nanobacteria - contaminated cells grow slowly, have poor cell states, and are severely vacuolated. They may even cause changes in cell morphology. Therefore, it is of great significance to clean up nanobacteria contamination in cell culture.

Small volumes of M199 culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2570343 is a culture media targeting M199. Also known as Nanobacteria Removal Medium (M199).