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> Phospho-Stat 1 (S727) and Total Stat 1 ELISA Kit

Phospho-Stat 1 (S727) and Total Stat 1 ELISA Kit

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Référence PEL-Stat1-S727-T-1

Conditionnement : 1PlateKit

Marque : RayBiotech


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Phospho-Stat 1 (S727) ELISA KitPhospho-Stat 1 (S727) ELISA Kit

Phospho-Stat 1 (S727) ELISA Kit

Phospho-Stat 1 (S727) ELISA Kit

The RayBiotech Phospho-Stat 1 (S727) ELISA Kit measures phosphorylated Stat 1 (Ser727) and total (pan) Stat 1 in cell and tissue lysates.
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Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman, Mouse
Specificity
The antibody pair provided in this kit recognizes human and mouse Phospho-Stat1 (pSer727).
Compatible Sample TypesTissue Lysates, Cell Lysates
Accession Number
P42224
Gene Id
6772
Gene Symbols
STAT1
Protein Name / Synonyms
Signal transducer and activator of transcription 1-alpha/beta (Transcription factor ISGF-3 components p91/p84)
Quantitative/Semi-QuantitativeSemi-Quantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaAngiogenesis, Cancer, Post-Translational Modifications, Phosphorylation, JAK/STAT Signaling
Shipping TypeBlue ice
Storage-20°C

Product Features

  • Simultaneously measure phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody (*not included in phospho-only version)
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 µl of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 µl of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

citations

  • C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells

    Species:
    Mouse
    Year:
    2017