Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders.

Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. Pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex=585/530nm is directly proportional to pyruvate concentration in the sample.

Key Features:
Sensitive and accurate. Use as little as 10ul samples. Linear detection range in 96-well plate: 2 to 500uM (17 ug/dL to 4.4 mg/dL) pyruvate for
Colorimetric Assays and 0.2 to 50uM for Fluorometric Assays.

Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.

Improved reagent stability: The optimized formulation has greatly enhanced the reagent and signal stability.

Applications:
Direct Assays: Pyruvate in biological samples
Drug Discovery/Pharmacology: Effects of drugs on pyruvate metabolism

Kit Components:
P9543-85A: Enzyme Mix, 1x10ml
P9543-85B: Dye Reagent, 1x120ul
P9543-85C: Standard (25mM Pyruvate), 1x400ul

Storage and Stability:
Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.