QuickTiter Wild Type Adenovirus Titer Immunoassay Kit

Background: Adenoviruses are non-enveloped viruses with icosahedral capsids of 90 to 100 nm in size containing a double stranded DNA genome ranging from 26 to 46 kb. Recombinant adenoviruses have been developed as gene delivery vectors for recombinant vaccines and gene therapy applications, including the treatment of metabolic disorders and cancers. Traditionally, infectivity particles are measured in culture by a plaque-forming unit assay (PFU) that scores the number of viral plaques as a function of dilution. These methods are time-consuming (10- 14 days), require a long infection period, and suffer from a high degree of inter-assay variability and are affected by virus-cell interactions. The QuickTiter™ Immunoassay Kit utilizes an antibody against adenoviral hexon proteins to visualize infected cells by immunocytochemistry staining. The hexon proteins are the largest and most abundant of the structural proteins in the adenovirus capsid, and they are distributed symmetrically to form capsid facets. The QuickTiter™ Immunoassay Kit provides a quick and complete system to functionally titer virus infectivity. The kit provides sufficient reagents for up to 100 titrations in a 24-well plate. In contrast to the 10 to 14-day infection of a classical plaque assay, the kit only requires a 2-day infection. The kit antibody against adenoviral hexon protein recognizes all 51 serotypes of adenovirus by immunocytochemistry and can be used with any adenovirus system as long as the virus is able to amplify in A549 or similar permissive cells.

Small volumes of Wild Type Adenovirus Titer kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS169635 is a kit targeting Wild Type Adenovirus Titer. Also known as QuickTiter Wild Type Adenovirus Titer Immunoassay Kit.