Rapid Clear Cap 3000, High-Speed Clarification of 2L-4L of Cell Culture, Sterile | Use w/ 931114, 931116

Référence 788116

Conditionnement : CS4

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A revolutionary new technology that allows high speed clarification of cellular material

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with no centrifugation required

Revolutionary Technology In Downstream Processing

The Thomson Optimum Growth® System of products is expanding into downstream processing with a revolutionary new technology that allows high speed clarification of cellular material. Thomson developed the Rapid Clear® Cap to address the needs of scientists to quickly and efficiently clarify cell culture media directly from the Thomson 5L Optimum Growth® shaker flask without the need for centrifugation.

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Key Features
  • Depth filtration with a 0.2μm final pore size eliminates multiple filtration steps and in most cases centrifugation

  • Significant time savings versus traditional spin down technique

  • Cell culture clarification of low or high density cultures of CHO stable, CHO transient, HEK293, hybridoma, and other mammalian cell lines

  • Eliminates transfer steps: The Rapid Clear® Cap screws directly onto the Optimum Growth® Flask

  • Secondary cap attaches to a new Optimum Growth® Flask or to a storage container with a Luer lock

Key Benefits
  • Save time, clarify 3L of cell culture in less than 30 minutes - with no centrifugation required!

  • Reduce consumables used by up to 90%

  • Walk away convenience and safety - minimize endotoxin exposure

Simply switch out the vented Optimum Growth® Cap for the Rapid Clear®. The only equipment required is a peristaltic pump. In initial testing the units can filter up to 2.5L-3L of high density culture, 6x10^6 -20x10^6 @ >70% viability, in approximately 18 minutes. This technique will transform the time consuming and laborious task of harvesting cells to a rapid walk away procedure.

with Faster Filtration!
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Time in Minutes

Clarify your cultures in under 30 minutes

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CHO & HEK293 Filtration

Quick cell harvest for mammalian cells

= Less Waste
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Current Method

  • 4 x filter funnels
  • 6 x containers
10 items
+ centerfugation needed
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New Method

  • 1 x Optimum Growth® Flask
  • 1 x Rapid Clear® Cap
Only 2 items needed
Rapid Clear® Cap 3000 to GE® Capsule Filters

HPLC was utilized to quantitate intact protein for each of the purified solutions. The Rapid Clear® System yielded a slightly higher quantity of intact IgG, than the GE capsule columns.

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HPLC comparison of the purified IgG. The Rapid Clear® Cap 3000 is the red line and GE the blue line

Both GE and Rapid Clear® clarified IgG were run on an SDS Page gel for comparison: Lanes 1 & 4 are molecular weight standard ladder; Lanes 2&3 are non-reduced; lanes 5&6 are reduced.

  • 1 ug of post proA protein per lane
  • Both Reduced and Non-reduced samples
  • 4-12% Bis Tris Gel from Life Tech
  • Run in MES buffer @ 200 V for 30 minutes
  • Stained with Safe Stain
  • Ladder is Precision Plus from Biorad

A= GE filtered material
B= Rapid Clear®filtered material

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Reduce Operating Costs by Increasing Efficiency & Minimizing the Number of Consumables Used

When producing biologics, cell yield and viability, and effective clarification are critical. Thomson's patented Optimum Growth® Flask design facilitates good mixing and high gas exchange rates to produce high density yields of viable cells. Thomson has used its expertise in filtration to develop the Rapid Clear® Cap 3000 to speed up the clarification process. This innovation reduces operating costs by increasing efficiency and minimizing the number of consumables used.

Quick & Easy to Use

Using the Rapid Clear® Cap 3000 is quick and easy. Once the mammalian or insect cell culture is ready for clarification, simply remove the vent cap and replace it with the Rapid Clear® Cap 3000. The chart provides the approximate time*** to clarify cell cultures based on cell type, viability and volume to be filtered. The higher the viability the faster the filtration.

  • Grow cells in a 2.8L or 5L Optimum Growth® Flask

  • Transfer the flask to a hood to replace the Vent Cap with the Rapid Clear® Cap

  • Replace the Vent Cap on the receiving 1.6L, 2.8L, or 5L Optimum Growth® Flask with the receiving cap that comes with the Rapid Clear® Cap

  • Remove from hood and place tubing in the pump head, see pump recommendations***

  • Tilt the flask slightly and run the pump at maximum speed until only a few hundred mL remain

  • Add 400mL of PBS to the Optimum Growth® Flask to ensure all the culture has been filtered and transferred to the new flask

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Duplicate 3L CHO Stable cell cultures were clarified using the Rapid Clear® 3000. This graph compares the volume clarified over 21 minutes.

Volume & time recommendations by cell type

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Référence
Description
Cond.
Prix HT
A13432-100
 100mg