GMP Recombinant Human IL-3 Protein is produced in GMP compliance facility designed under ISO 13485:2016, cGMP, and ISO 17025:2017 certifications utilizing animal-origin free and beta-lactam free materials under stringent quality management.
GMP Grade
Recombinant Human IL-3 Protein, GMP Grade
Recombinant Human IL-3 Protein, GMP Grade
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Species | Human |
| Accession Number | P08700 |
| Gene Id | 3562 |
| Gene Symbols | IL3 |
| Protein Name / Synonyms | Interleukin-3 (IL-3) (Hematopoietic growth factor) (Mast cell growth factor) (MCGF) (Multipotential colony-stimulating factor) (P-cell-stimulating factor) |
| Expressed Region | Ala20-Phe152 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The recombinant human IL-3 protein has predicted molecular weight of 15 kDa. Due to glycosylation, the protein migrates as 17-30 kDa on SDS-PAGE gel under reducing conditions. |
| Format | White powder |
| Formulation | Lyophilized powder from sterile filtered PBS, pH 7.4, with stabilizers. |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5-1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | < 10 EU per mg of protein as determined by the LAL method |
| Microbial Test | Negative |
| Research Area | Inflammation, cAMP/cGMP Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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SDS-PAGE Image
Activity
Host Cell DNA
Lot specific. Contact us for information.
Host Cell Protein
0.51 ng/µg of protein tested by ELISA
Mycoplasma
Negative
Usage
GMP-grade reagent. Not for direct human use.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Description
Interleukin 3 (IL-3) is a multilineage hematopoietic cytokine that regulates the proliferation, differentiation, and survival of various myeloid progenitor cells. It is primarily produced by activated T cells, mast cells, and endothelial cells in response to immune stimuli. It plays a crucial role in immune responses, inflammation, and hematopoiesis by enhancing the survival and function of immune cells. GMP Recombinant Human IL-3 Protein is produced in GMP compliance facility designed under ISO 13485:2016, cGMP, and ISO 17025:2017 certifications utilizing animal-orign free and beta-lactam free materials under stringent quality management.