RH PDGF-AA (AF)
Référence 100-16AF-10UG
Conditionnement : 10UG
Marque : FUJIFILM
Recombinant Human PDGF-AA
Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.
Also available as a GMP product: CTGrade GMP rh PDGF-AA
Product images on the website are not representative of actual product, including, but not limited to differences in cap color, tube type, or label format.
SDS-PAGE Showing a Single Prominent Band at 32 kDa
Figure 1. rh PDGF-AA (1μg) was resolved with SDS-PAGE under reducing (R) conditions and visualized by InstantBlue staining showing a single prominent band at 32 kDa. Predicted Molecular weight is 28.9 kDa but runs on SDS-PAGE at ~32 kDa.
Cell-Based Proliferation Assay
Figure 2. Recombinant Human PDGF-AA induced proliferation in NR6R/3T3 cells.
Instructions
Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Technical Specifications
| Alternate Names | GDGF, ODGF |
| Accession Number | P04085 |
| Source | Genetically modified E.coli |
| Predicted Molecular Mass | Dimer, 14.4/28.9 kDa (126/252 aa) |
| AA Sequence | MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 0.1% Trifluoroacetic Acid (TFA) |
| Product Reconstitution | Sterile water at 0.1 mg/mL |
| Country of Origin | USA |
| Specification* | Method of Determination | Acceptance Criteria |
| Purity | Reducing and Non-Reducing SDS-PAGE | ≥ 95% |
| Endotoxin | Kinetic LAL | ≤ 0.1 EU/µg |
| Biological Activity (ED50) | NR6R-3T3 cell proliferation | ≤ 50 ng/mL; ≥ 2.0 x 104 units/mg |
*Lot-specific values for the above specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.