- More Files
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human lung cancer (FFPE) stained with ROS1 Split FISH Probe. Human lung cancer showed ROS1 gene split.
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Huma lung, adenosquamous cell carcinoma (FFPE) stained with ROS1 Split FISH Probe. Human lung, adenosquamous cell carcinoma showed no ROS gene split.
Hybridization position of the probes on the chromosome:
Hybridization position of the probes on the chromosome:
- Specification
Product Description
Labeled FISH probes for identification of gene split using Fluorescent In Situ Hybridization Technique. ().
Probe 1
Name: ROS1(Texas Red)
Size: Approximately 250kb
Fluorophore: Texas Red
Location: 6q22.1Probe 2
Name: ROS1(FITC)
Size: Approximately 720kb
Fluorophore: FITC
Location: 6q22.1Probe Gap
The length of overlap between two probes is approximately 60 kb.
Origin
Human
Source
Genomic DNA
Reactivity
Human
Form
Liquid
Notice
We strongly recommend the customer to use FFPE FISH PreTreatment Kit 1 (Catalog #: KA2375 or KA2691) for the pretreatment of Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Regulation Status
For research use only (RUO)
Quality Control Testing
Representative images of normal human cell (lymphocyte) stain with the dual color FISH probe. The left image is chromosomes at metaphase, and the right image is an interphase nucleus.
Supplied Product
DAPI Counterstain (1500 ng/mL ) 125 uL for each 100 uL FISH Probe
Storage Instruction
Store at 4°C in the dark.
Note
Hybridization position of the probes on the chromosome:
- Applications
Fluorescent In Situ Hybridization (Cell)
Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Human lung cancer (FFPE) stained with ROS1 Split FISH Probe. Human lung cancer showed ROS1 gene split.Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections)
Huma lung, adenosquamous cell carcinoma (FFPE) stained with ROS1 Split FISH Probe. Human lung, adenosquamous cell carcinoma showed no ROS gene split. - Gene Info — ROS1
Entrez GeneID
6098Gene Name
ROS1
Gene Alias
MCF3, ROS, c-ros-1
Gene Description
c-ros oncogene 1 , receptor tyrosine kinase
Omim ID
165020Gene Ontology
HyperlinkGene Summary
This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The protein encoded by this gene is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor. [provided by RefSeq
Other Designations
OTTHUMP00000017814|OTTHUMP00000040389|proto-oncogene c-ros-1 protein|proto-oncogene tyrosine-protein kinase ROS|transmembrane tyrosine-specific protein kinase|v-ros UR2 sarcoma virus oncogene homolog 1|v-ros avian UR2 sarcoma virus oncogene homolog 1
- Interactome
- Disease
- Publication Reference
- Expression levels of ROS1/ALK/c-MET and therapeutic efficacy of cetuximab plus chemotherapy in advanced biliary tract cancer.
Chiang NJ, Hsu C, Chen JS, Tsou HH, Shen YY, Chao Y, Chen MH, Yeh TS, Shan YS, Huang SF, Chen LT.
Amino Acids 2016 May; 6:25369.
Application：FISH, Human, Primary intra-hepatic cholangiocarcinoma.
- Identification of ROS1 rearrangement in gastric adenocarcinoma.
Lee J, Lee SE, Kang SY, Do IG, Lee S, Ha SY, Cho J, Kang WK, Jang J, Ou SH, Kim KM.
Cancer 2013 Feb; 119(9):1627.
Application：FISH, Human, Gastric adenocarcinoma.
- ROS1 receptor tyrosine kinase, a druggable target, is frequently overexpressed in non-small cell lung carcinomas via genetic and epigenetic mechanisms.
Lee HJ, Seol HS, Kim JY, Chun SM, Suh YA, Park YS, Kim SW, Choi CM, Park SI, Kim DK, Kim YH, Jang SJ.
Annals of Surgical Oncology 2013 Jan; 20(1):200.
Application：FISH, Human, Human non-small cell lung carcinomas.
- Expression levels of ROS1/ALK/c-MET and therapeutic efficacy of cetuximab plus chemotherapy in advanced biliary tract cancer.