Applications:

• Affinity matrix with immobilized Streptavidin by amino coupling method.
• The base matrix is a rigid, highly cross-linkedbeaded agarose with high chemical stability.
• The immobilized streptavidin bindsbiotin and biotinylated substances and can be used for affinity chromatographyapplications.
• The interaction between streptavidin and biotin is very strong andrequires denaturating conditions for elution, which may destroy both the ligandand the sample.
• Strptavidin-Separopore® 4B is extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin.
• It can be used in the purification of antigens, where biotinylatedantibodies are incubated with antigen. The biotinylated antibody-antigencomplex binds to Streptavidin Separopore from which the antigen can be eluted.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:

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