T4 Polynucleotide Kinase Reaction Buffer
Catalog Number: B2019317 (4 mL)
T4 Polynucleotide Kinase Reaction Buffer is a buffer used in molecular biology for the phosphorylation of nucleic acids. It provides optimal conditions for T4 polynucleotide kinase activity, facilitating the transfer of a phosphate group from ATP to the 5′ end of nucleic acid molecules. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
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Product Description
T4 Polynucleotide Kinase Reaction Buffer
Catalog number: B2019317
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 4 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20°C
Keywords: T4 Polynucleotide Kinase Reaction Buffer
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: Chai Y, Cheng X, Xu G, Wei F, Bao J, Mei J, Ren D, Hu Q, Cen Y. A nanoplatform based on metal-organic frameworks and coupled exonuclease reaction for the fluorimetric determination of T4 polynucleotide kinase activity and inhibition Mikrochim Acta. 2020 Mar 23;187(4):243.
2: Zhang H, Zhao Z, Lei Z, Wang Z. Sensitive Detection of Polynucleotide Kinase Activity by Paper-Based Fluorescence Assay with λ Exonuclease Assistance Anal Chem. 2016 Dec 6;88(23):11358-11363.
3: Cui L, Hu J, Li CC, Wang CM, Zhang CY. An electrochemical biosensor based on the enhanced quasi-reversible redox signal of prussian blue generated by self-sacrificial label of iron metal-organic framework Biosens Bioelectron. 2018 Dec 30;122:168-174.
4: Holden NS, Tacon CE. Principles and problems of the electrophoretic mobility shift assay J Pharmacol Toxicol Methods. 2011 Jan-Feb;63(1):7-14.
5: Folk WR. Phage T4 polynucleotide kinase Gene Amplif Anal. 1981;2:299-311.
6: Tao J, Liu Z, Zhu Z, Zhang Y, Wang H, Pang P, Yang C, Yang W. Electrochemical detection of T4 polynucleotide kinase activity based on magnetic Fe(3)O(4)@TiO(2) nanoparticles triggered by a rolling circle amplification strategy Talanta. 2022 May 1;241:123272.
7: Lim RC, Gary RK. Kinetic analysis of T4 polynucleotide kinase via isothermal titration calorimetry Arch Biochem Biophys. 2024 Jun;756:109995.
8: Wu Y, Yi J, Su A, Zhang Y, Wang H, Yang L, Yang W, Pang P. An electrochemical biosensor for T4 polynucleotide kinase activity identification according to host-guest recognition among phosphate pillar[5]arene@palladium nanoparticles@reduced graphene oxide nanocomposite and toluidine blue Mikrochim Acta. 2023 Sep 16;190(10):394.
9: Wei L, Kong X, Wang M, Zhang Y, Pan R, Cheng Y, Lv Z, Zhou J, Ming J. A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation Anal Bioanal Chem. 2022 Nov;414(27):7923-7933.
10: Jiang Y, Cui J, Zhang T, Wang M, Zhu G, Miao P. Electrochemical detection of T4 polynucleotide kinase based on target-assisted ligation reaction coupled with silver nanoparticles Anal Chim Acta. 2019 Nov 28;1085:85-90.
Products Related to T4 Polynucleotide Kinase Reaction Buffer can be found at Chemicals
Additional Information
| Weight | 0.8 oz |
|---|---|
| Dimensions | 2 × 0.9 × 0.9 in |