T7 RNA Polymerase, GMP Grade
Référence GMP-E121-HC-M001
Conditionnement : 1000KU
Marque : Novoprotein
Description - As a biological macromolecule, mRNA can be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is one of the most efficient promoters at present. Therefore, T7 RNA polymerase can be used for in vitro transcription to obtain more synthetic products. T7 RNA polymerase is a T7 promoter-specific, DNA-dependent, 5'→3' RNA polymerase from T7 bacteriophage. Using double stranded DNA as the template, it transcribes RNA complementary to the single stranded DNA located at the downstream of T7 promoter. T7 RNA polymerase has been commonly used for in vitro mRNA synthesis.
- The polymerase is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
- This product has completed the DMF record of FDA and passed the HALAL certification.
Quality Control | Element | Standard |
|---|---|
| Appearance | Clear and transparent solution |
| Identification | Positive |
| Visible Particles | Meet the specification |
| pH | 7.5-8.5 |
| Activity | 980KU/ml-1020KU/ml |
| Purity | ≥95% |
| Endonuclease Residues | The degradation of substrate was ≤10% |
| Exonuclease Residues | The degradation of substrate was ≤10% |
| RNase Residues | The degradation of substrate was ≤10% |
| Bacterial Endotoxins | ＜10EU/mg |
| Exogenous DNA Residues | ≤100pg/mg |
| Host-cell Protein Residues | ≤50ppm |
| Mycoplasma | Negative |
| Heavy Metals | ≤10ppm |
| Microbial Limit | Total aerobic microbial count≤1cfu/10ml, total yeasts and molds count≤1cfu/10ml |
Components | SKU | Components | Volume |
|---|---|---|
| GMP-E121-HC-U010 | T7 RNA Polymerase, GMP Grade (1000U/μl) | 10μl |
| GMP-E121-HC-U100 | T7 RNA Polymerase, GMP Grade (1000U/μl) | 100μl |
| GMP-E121-M001 | T7 RNA Polymerase, GMP Grade (1000U/μl) | 1ml |
| GMP-E121-M010 | T7 RNA Polymerase, GMP Grade (1000U/μl) | 10ml |
| GMP-E121-M050 | T7 RNA Polymerase, GMP Grade (1000U/μl) | 50ml |
Storage At -20±5℃.
Applications 
Fig: Transcription of RNA in vitro Annotation: 1) From left to right, the quantity of T7 RNA Polymerase copies were 20 U, 4 U, 0.8 U. 2) DNA templates were 2Kb fragment. Related Products | Cat. No. | Product | Cat. No. | Product |
|---|---|---|---|
| GMP-M062 | Vaccinia Capping System, GMP Grade | GMP-E125 | RNase Inhibitor, GMP Grade |
| GMP-M072 | mRNA Cap 2´-O-Methyltransferase, GMP Grade | GMP-E127 | DNase I, GMP Grade |
| GMP-M012 | E. coli Poly(A) Polymerase, GMP Grade | GMP-M036 | Pyrophosphatase, Inorganic (yeast), GMP Grade |
| GMP-EB231 | 10×Transcription buffer, GMP Grade | GMP-E131 | T7 RNA Transcription Enzyme Mix, GMP Grade |
| GMP-S023-026 | NTPs, GMP Grade | ||