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> Taq DNA Polymerase (Buffer with Mgcl2)

Taq DNA Polymerase (Buffer with Mgcl2)

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Référence MBS355980-5x500U

Conditionnement : 5x500Units

Marque : MyBiosource

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Taq DNA Polymerase (Buffer with Mgcl2) Reagent

Taq DNA Polymerase (Buffer with Mgcl2) reagent
Product Name

Taq DNA Polymerase (Buffer with Mgcl2), Reagent

Full Product Name

Taq DNA Polymerase (Buffer with Mgcl2)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS355980 COA
COA PDF
MBS355980 Testing Data
Testing Data PDF
MBS355980 QC Report
QC Report PDF
MBS355980 MSDS
MSDS PDF
MBS355980 Technical Datasheet
Technical Datasheet PDF
MBS355980 Protocol
Protocol PDF
Activity Definition
The activity of 1 unit (U) of Taq DNA Polymerase is defined as at 74 degree C for 30 min, use activated salmon sperm DNA as template primer, then add 10nmol of deoxynucleotides to the amount of enzyme required for acid-insoluble substances.
Enzyme Storage Buffer
20mM Tris-HCl (pH8.0), 0.1 mM EDTA, 1mM DTT, 100 mM KCl, Stabilizers, 50% glycerol.
10xTaq Buffer (with Mg2+):200 mM Tris-HCl (pH 8.4), 200 mM KCl, 100 mM(NH4)2 SO4, 15 mM MgCl2, and other components.
a. 10x Taq Buffer is divided into Mg2+ and without Mg2+, the user can choose.
b. Buffer without Mg2+, will be equipped with 25 mM MgCl2 additionally.
c. If not specified, it is usually provided that contains Mg2+ Buffer.
Other Notes
Small volumes of Taq DNA Polymerase (Buffer with Mgcl2) reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
Taq DNA Polymerase (Buffer with Mgcl2) reagent
Taq DNA Polymerase is isolated and purified from E Coli cloned with Thermu aquaticus DNA Polymerase gene after induction table. Its molecular weight is 94 KD. Taq DNA Polymerase has 5'-3' DNA polymerase activity and 5'-3' exonuclease activity, but no 3'-5' exonuclease activity. In the PCR reaction, the extension speed of Taq DNA Polymerase is 1-2kb/min, and the product with A at the 3'end, which can be directly used for T/A vector cloning.
Product Categories/Family for Taq DNA Polymerase (Buffer with Mgcl2) reagent
PCR and RT-PCR; PCR Related Products
Sample Manual Insert of MBS355980.