Biological Activity

We have demonstrated, to the best of our knowledge for the first time, the effects of short (1-day) and prolonged (2-weeks, 10-weeks) in vivo administrations of testosterone enanthate (TE) on molecular markers of pituitary functionality as well as prolactin (PRL) and cAMP signaling in Leydig cells.

Protocol (for reference only)

Cell Experiment
Cell lines	Leydig cells
Preparation method	For in vitro experiments the primary cultures of purified Leydig cells were isolated from undisturbed rats and plated for monitoring genes expression. After 2 h of recovery, the culture medium was trashed and the Leydig cells were incubated for 20 h without (CUT, untreated (control) cells) or with test osterone (T, 20 μM ) or cyproterone-acetate (CPA, 100 μM ) or combination CPA (100 μM ) + T (20 μM ), or in presence of hCG (5 192 and 50 ng/ml) as a positive control.
Concentrations	20 μM
Incubation time	20 h

Animal Experiment
Animal models	male Wistar rats
Formulation
Dosages	0.5mg/100g or 2.5 mg/100g
Administration	i.v.

Chemical Information

Molecular Weight	400.59
Formula	C26H40O3
CAS Number	315-37-7
Solubility (25°C)	10 mM in DMSO
Storage	Powder          -20°C   3 years ;  4°C   2 years In solvent       -80°C   6 months ;  -20°C   1 month

References

[1] Bjelic MM, et al. J Steroid Biochem Mol Biol. Prolonged in vivo administration of testosterone-enanthate, the widely used and abused anabolic androgenic steroid, disturbs prolactin and cAMP signaling in Leydig cells of adult rats.

[2] Bjelic MM, et al. Mol Cell Endocrinol. Molecular adaptations of testosterone-producing Leydig cells during systemic in vivo blockade of the androgen receptor.