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Thapsigargin [67526-95-8]

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Référence NB-64-69348-1mg

Conditionnement : 1mg

Marque : Neo Biotech

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Thapsigargin

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Catalog No. TQ0302 Copy Product Info

Purity: 99.70%

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Thapsigargin is a natural product, an inhibitor of sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) and an endoplasmic reticulum stress inducer. Thapsigargin increases cytoplasmic calcium concentration by blocking the ability of cells to pump calcium into the sarcoplasmic and endoplasmic reticulum.

Cas No. 67526-95-8

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.70%

Appearance:Solid

Color:White

Product Introduction

Bioactivity

Description	Thapsigargin is a natural product, an inhibitor of sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) and an endoplasmic reticulum stress inducer. Thapsigargin increases cytoplasmic calcium concentration by blocking the ability of cells to pump calcium into the sarcoplasmic and endoplasmic reticulum.
Targets & IC50	EL4 cells:0.1 μM, CCRF-CEM cells:0.27 μM, HL-60 cells:0.007 μM, PC3 cells:2.4 μM, CCRF S-180 cells:30 nM, K562 cells:0.17 μM, MCF-7 cells:2.3 μM
In vitro	METHODS: Human rheumatoid arthritis synoviocytes MH7A were treated with Thapsigargin (0.001-1 μM) for 2-4 days, and cell proliferation was detected using the SRB. RESULTS: Thapsigargin inhibited the proliferation of MH7A cells in a time- and dose-dependent manner. [1] METHODS: Human hepatocellular carcinoma cells HepG2 were treated with Thapsigargin (25-100 nM) for 24 h. Endoplasmic reticulum stress/UPR gene expression was detected by RT-qPCR. RESULTS: Thapsigargin treatment consistently induced ER stress gene expression only at elevated concentrations of 50 and 100 nM. [2]
In vivo	METHODS: To assay in vivo ER stress-inducing activity, Thapsigargin (0.25-1 μg/g in 150 mM dextrose containing 1% DMSO) was administered as a single intraperitoneal injection to Balb/c mice in order. RESULTS: Thapsigargin treatment resulted in significant expression of the ER stress markers ATF6 and eIF2α in adipose tissue. thapsigargin treatment failed to induce the expression of most of the ER stress and UPR proteins in the liver. [2] METHODS: To investigate the antiviral function in vivo, Thapsigargin (30 ng/mouse) was administered by gavage to PR8 virus-infected BALB/c mice once daily for seven days. RESULTS: Oral administration of Thapsigargin to mice significantly reduced severity and viral shedding and improved survival during infection with the deadly influenza virus. [3]
Cell Research	Cell Line: MH7A human rheumatoid arthritis synovial cells. Concentration: 0.001, 0.1, and 1?μM. Incubation Time: For 2 and 4 days [2]
Animal Research	Animal Model: Male Balb/c mice (20-25g). Dosage: 0.25ug/g, 0.5ug/g and 1ug/g. Administration: Injection; 24 hours [4]

Chemical Properties

Molecular Weight	650.75
Formula	C₃₄H₅₀O₁₂
Cas No.	67526-95-8
Smiles	O(C(CCCCCCC)=O)[C@H]1[C@]2(C([C@]3([C@](O)([C@@H](OC(CCC)=O)C[C@@]2(OC(C)=O)C)[C@](C)(O)C(=O)O3)16)=C(C)[C@@H]1OC(/C(=C\C)/C)=O)16
Relative Density.	1.1521 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Store at low temperature,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 252.5 mg/mL (388.01 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (7.68 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.5367 mL	7.6834 mL	15.3669 mL	76.8344 mL
5 mM	0.3073 mL	1.5367 mL	3.0734 mL	15.3669 mL
10 mM	0.1537 mL	0.7683 mL	1.5367 mL	7.6834 mL
20 mM	0.0768 mL	0.3842 mL	0.7683 mL	3.8417 mL
50 mM	0.0307 mL	0.1537 mL	0.3073 mL	1.5367 mL
100 mM	0.0154 mL	0.0768 mL	0.1537 mL	0.7683 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

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Keywords

unfoldedThapsigarginstressSARS-CoVSARSCoVSARS coronavirusprotein ,responseInhibitorinhibitcoronavirusCalciumChannelCalcium ChannelCa2+-ATPaseCa2+ channelsCa channelsApoptosis

Related Tags: Thapsigargin chemical structure | Thapsigargin in vivo | Thapsigargin in vitro | Thapsigargin formula | Thapsigargin molecular weight