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> Tributyrin [60-01-5]

Tributyrin [60-01-5]

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Référence HY-W011404-5mL

Conditionnement : 5mL

Marque : MedChemExpress


Description

Tributyrin (Glyceryl tributyrate), a neutral short-chain fatty acid triglyceride, is a stable and rapidly absorbed proagent of Butyric Acid. Tributyrin diffuses through biological membranes and is metabolized by intracellular lipases, releasing effective butyrate directly into the cell in vivo. Tributyrin has potent antiproliferative, proapoptotic and differentiation-inducing effects[1].

IC50 & Target

IL-1β

 

In Vitro

Tributyrin (0.8 mM, 48 hours) induces significant growth inhibition in PC-3 cells[3].
Tributyrin (0.5-5 mM, 48 hours) causes G1 cell cycle arrest in LNCaP cells[3].
Tributyrin (0.1-5 mM, 48 hours) induces higher apoptosis rates in PC-3 and TSU-PR1 cells[3].
Tributyrin (1-5 mM, 48 hours) induces prostate-specific antigen expression in LNCaP cells, suggesting its differentiation effect[3].
Tributyrin (1 mM, 24 hours) directly inhibits the production of TNF-α and IL-1β in RAW 264.7 cells under LPS stimulation[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tributyrin Related Antibodies

Cell Proliferation Assay[3]

Cell Line: LNCaP, PC-3, TSU-PR1 cells (human prostate cancer)
Concentration: 0.1-5 mM
Incubation Time: 48 hours
Result: Exhibited significant growth inhibition in all three cell lines, with IC50 of 0.8 mM in PC-3 cells, 1.2 mM in TSU-PR1 cells, and 3.1 mM in LNCaP cells.

Cell Cycle Analysis[3]

Cell Line: LNCaP cells (human prostate cancer)
Concentration: 0.5, 2, 5 mM
Incubation Time: 48 hours
Result: Significantly reduced the fraction of LNCaP cells in the S-phase and caused strong accumulation in the G1 phase.
In Vivo

Tributyrin (2.0 g/kg, p.o., three times per week for 6 weeks) improves the metabolic and inflammatory states in high-fat diet-induced obese mice[4].
Tributyrin (0.3 g/kg, p.o., once daily for 11 days) significantly improves gut microbiota dysbiosis and intestinal injury in a Ceftriaxone (HY-B0712)-induced gut dysfunction mouse model[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: High-fat diet (HFD)-induced obese mice (C57BL/6)[4]
Dosage: 2.0 g/kg
Administration: Oral gavage (p.o.), 3 times per week, with 48-hour intervals, for 6 weeks
Result: Led to lower body weight gain compared to control, improved glucose tolerance, increased insulin sensitivity, and reduced liver triglyceride content.
Animal Model: Ceftriaxone (HY-B0712)-induced gut microbiota dysbiosis in C57BL/6 mice
Dosage: 0.3 g/kg, 3 g/kg
Administration: Oral gavage (p.o.), once daily, for 11 days
Result: Improved body weight, reduced diarrhea, and less intestinal tissue damage (0.3 g/kg).
Masse moléculaire

302.36

Formule

C15H26O6
CAS No.
Appearance

Liquid (Density: 1 g/ml)

Color

Colorless to light yellow

SMILES

CCCC(OCC(OC(CCC)=O)COC(CCC)=O)=O
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvant et solubilité
In Vitro: 

DMSO : 100 mg/mL (330.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3073 mL 16.5366 mL 33.0732 mL
5 mM 0.6615 mL 3.3073 mL 6.6146 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Pureté et documentation
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