Triiodothyronine indes, Free (FT3) BioAssay™ ELISA Kit (Fish)

Sample Type:
Serum and plasma

Intended Use:
For the quantitative determination of fish free tri-iodothyronine indes(FT3) concentrations in serum and plasma.

Sensitivity:
1.9pg/ml

Range:
10-160pg/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of fish FT3. No significant cross-reactivity or interference between fish FT3 and analogues was observed.

Test Principle:
This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FT3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated FT3. A competitive inhibition reaction is launched between FT3 (Standards or samples) and Biotin-conjugated FT3 with the pre-coated antibody specific for FT3. The more amount of FT3 in samples, the less antibody bound by Biotin-conjugated FT3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of FT3 in the sample. The color development is stopped and the intensity of the color is measured.

Kit Components:
Assay plate, 1x96 wells
Standard, 5x1ml
Conjugate, 1x6ml
HRP-avidin, 1x6ml
Wash Buffer (20X), 1x15ml
Substrate A, 1x7ml
Substrate B, 1x7ml
Stop Solution, 1x7ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.