XTT Cell Proliferation Assay Kit is a widely used cell proliferation and toxicity assay kit. XTT, a novel tetrazolium salt, can be reduced by succinate dehydrogenase in the mitochondria of living cells to the water-soluble formazan crystals. The amount of formazan can be quantified by measuring the absorbance at 450 nm upon solubilization and is proportional to the number of living cells.

Compared with the widely used MTT Assay Kit, this kit does not need to remove the culture medium, nor to use a solvent to dissolve the formazan, which is more convenient to use, and can avoid the experimental error caused by taking away part of the formazan when removing the medium. The kit is very flexible and can be continuedly incubated after detection for better color rendering. At the same time, this kit is safe for use, highly sensitive and has a good linear range.

Figure 1: Reference standard curve of this kit