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> ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (Biotin)

ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (Biotin)

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Référence Z0750-02B-Biotin-100ul

Conditionnement : 100ul

Marque : US Biological


Z0750-02B-Biotin ZO-1 (Tight Junction-associated Polypeptide, Zonula Occludens 1) (Biotin)

Clone Type
Polyclonal
Host
rabbit
Source
human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Ca Gp Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

ZO-1 is a peripheral membrane protein that is associated with tight junctions (zonula occludentes) which are found on the cytoplasmic surface of epithelial and endothelial cell membranes. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as 'motif-a'. These two isoforms are the basis for determining two classes of tight junctions. ZO-1 is known to interact with ZO-2. ZO-1 is homologous to dlg, the Drosophila tumor suppressor. Both are members of a putative signal transduction protein family whose members contain a SH3 domain and a domain homologous to the enzyme guanylate kinase.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:.
Immunohistochemistry: Frozen sections
Immunohistochemistry: For formalin fixed, paraffin embedded tissue sections. Protease XIV pretreatment is required
Optimal dilutions to be determined by the researcher.

Positive Controls for Immunoprecipitation:
Canine MDCK and human Caco-2 cells.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.|Purity: Purified by immunoaffinity chromatography.|Immunogen: A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing.|Specificity: Recognizes human ZO-1 alpha+ and ZO-1 alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western Blots, but potential reactivity with these proteins has not been fully characterized. Species Crossreactivity: mouse, rat, guinea pig, canine.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ZO-1 alpha+ and ZO-1 alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western Blots, but potential reactivity with these proteins has not been fully characterized. Species Crossreactivity: mouse, rat, guinea pig, canine.
References
1. Willott, E. et al., Am J Physiol. 262 (Cell Physiol. 31):C1119-C1124 (1992). 2. Willott, E. et al., Proc. Natl. Acad. Sci., USA 90(16):7834-7838 (1993). 3. Balda, M.S. and Anderson, J.M., Am J Physiol 264 (Cell Physiol. 33):C918-C924 (1993). 4. Tsarfaty, I, et al., Science 263:98-101 (1994). 5. Keon, BH, et al., J. Cell Biol. 134(4):1003-1018 (1996). 6. Bomsel, M. Nature Medicine 3(1):42-47 (1997). 7. Watabe-Uchida, M, et al., J. Cell Biol 142(3):847-857 (1998). 8. Hoover, KB, et al., J. Am. J. Pathol 153(6):1767-1773 (1998). 9. Rajasekaran, AK, et al., J. Cell Biol 132(3):451-463 (1996). 10. Haskins, J, et al., J. Cell Biol. 141(1):199-208 (1998). 11. Kachinsky, AM, et al., J. Cell Biol. 145:391-402 (1999). 12. Wu, S., et al., J. Proc. Natl. Acad. Sci. 95(25):14979-14984 (1998). 13. Moreira, JE, et al. J. Histochem. Cytochem. 46(7):847-854 (1998).14. Potempa, S. and Ridley, A.J. Mol Biol Cell 9:2185-2200 (1998).15. Mann, B, et al., Proc. Natl. Acad. Sci. 96:1603-1608 (1999).16. Laing, JG, et al., J. Biol. Chem. 276 (25) : 23051-23055 (2001). 17. Hirata, K., et al., J. Biol. Chem. 276 (19) : 16223-16231 (2001).18. Toyofuku, T., et al., J. Biol. Chem. 276 (3) : 1780-1788 (2001).19. Singh, U., et al., J. Biol. Chem. 275 (24): 18407-18417 (2000).20. Bazzoni, G., et al., J. Biol. Chem. 275 (27) : 20520-20526 (2000).21. Fluge, O., et al., World J. Surg. 25: 623-631 (2001).22. Hamazaki, Y., et al., J. Biol. Chem. 277 (1): 455-461 (2002).23. Duffy, H.S., et al., J. Neuroscience 20 :RC114(1-6)