Orb1217442-500mg | 2,4-Dichlorophenoxyacetic acid [94-75-7] Clinisciences

2,4-Dichlorophenoxyacetic acid

SKU: orb1217442

Description

2,4-Dichlorophenoxyacetic acid is a synthetic auxin used as a plant growth regulator and also used as a supplement in plant cell culture media. It is an active ingredient in herbicides used to disrupts the actin cytoskeleton for the control of root elongation and cell production.

Images & Validation

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Key Properties

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CAS Number	94-75-7
MW	221.03
Purity	>98% (HPLC)
Formula	C₈H₆Cl₂O₃
SMILES	C1=CC(=C(C=C1Cl)Cl)OCC(=O)O
Target	DNA/RNA Synthesis
Solubility	DMSO:100 mg/mL (452.41 mM; Need ultrasonic)

Bioactivity

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In Vivo	Animal model: Adult Wistar male rats. Dosage: 100 or 200 mg/kg. Administration: i. g. Result: Decreased the testis, seminal vesicles and prostate relative weight. Decreased the number of spermatozoa and sperm motility. Increased the level of FSH and LH.
In Vitro	Cell Viability Assay Cell line: A549, W138. Concentration: 25, 50, 75, 100, 150, 200 μM. Incubation time: 72 h. Result: Inhibited cell viability in a dose-dependent manner. Cell Cycle Analysis Cell line: A549. Concentration: 50, 100, 150 μM. Incubation time: 72 h. Result: The G0/G1 population decreased to about 57%, 43% and 31%, respectively. Apoptosis Analysis Cell line: A549, W138. Concentration: 100, 150 μM. Incubation time: 72 h. Result: The early apoptotic population increased to 44% in A549 cells and 57% in WI38 cells, and the late apoptotic population increased to 18% in A549 cells and 10% in WI38 cells. Western blot analysis. Cell line: A549, W138. Concentration: 100, 150 μM. Incubation time: 72 h. Result: Up-regulated p53 and Bax proteins, down-regulated Bcl-2 and procaspase 3.

In Vivo

Animal model: Adult Wistar male rats. Dosage: 100 or 200 mg/kg. Administration: i. g. Result: Decreased the testis, seminal vesicles and prostate relative weight. Decreased the number of spermatozoa and sperm motility. Increased the level of FSH and LH.

In Vitro

Cell Viability Assay Cell line: A549, W138. Concentration: 25, 50, 75, 100, 150, 200 μM. Incubation time: 72 h. Result: Inhibited cell viability in a dose-dependent manner. Cell Cycle Analysis Cell line: A549. Concentration: 50, 100, 150 μM. Incubation time: 72 h. Result: The G0/G1 population decreased to about 57%, 43% and 31%, respectively. Apoptosis Analysis Cell line: A549, W138. Concentration: 100, 150 μM. Incubation time: 72 h. Result: The early apoptotic population increased to 44% in A549 cells and 57% in WI38 cells, and the late apoptotic population increased to 18% in A549 cells and 10% in WI38 cells. Western blot analysis. Cell line: A549, W138. Concentration: 100, 150 μM. Incubation time: 72 h. Result: Up-regulated p53 and Bax proteins, down-regulated Bcl-2 and procaspase 3.

Storage & Handling

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Storage	Storage temperature: -20°C. Stability: ≥ 2 years
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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2,4-D acid | Chloroxone | Chloroxone | Macondray | Hedonal

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2,4-Dichlorophenoxyacetic acid/2,4-D Antibody [orb3152047]
Applications:
ELISA
Species/Host:
Mouse
Conjugation:
Unconjugated
Sizes Available: 50 μg, 100 μg, 1 mg
2,4-Dichlorophenoxyacetic acid/2,4-D Antibody [orb3152053]
Applications:
ELISA
Species/Host:
Mouse
Conjugation:
Unconjugated
Sizes Available: 50 μg, 100 μg, 1 mg
2,4-Dichlorophenoxyacetic acid/2,4-D Nanobody Antibody [orb3152054]
Applications:
ELISA
Species/Host:
Camelus
Conjugation:
Unconjugated
Sizes Available: 50 μg, 100 μg, 1 mg
2,4-D-KLH conj. [orb86076]
Sizes Available: 1 mg
2,4-D-BSA conj. [orb86075]
Sizes Available: 1 mg