5×1 ml
2×TransTaq-T PCR SuperMix (+dye)
Referência AS122-11
Tamanho : 1ml
Marca : TransGen Biotech
Product Details
TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs, and optimized buffer. The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers, and H₂O. The amplification products feature 3′-dA overhangs, with A-addition efficiency equivalent to that of EasyTaq® DNA Polymerase. The enzyme offers high fidelity (comparable to EasyPfu DNA Polymerase) and high amplification efficiency. Amplicons obtained with 2× TransTaq®-T PCR SuperMix (+dye) can be directly loaded onto gels for electrophoresis; however, if cloning is required, the dye must be removed by purification prior to cloning.
• TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• High A‑addition efficiency for high‑efficiency cloning.
• Amplification of genomic DNA fragment up to 8 kb.
Applications
• Complex templates
• TA cloning
Storage
-18℃ or below for two years
Shipping
Dry ice (-70 ℃)
Product Composition
| Component | AS122-11 | AS122-12 |
| 2×TransTaq®-T PCR SuperMix (-dye)/(+dye) | 1 ml | 5×1 ml |
| Nuclease-free Water | 1 ml | 5 ml |
References
Li D M , Hou Y , Song X P , et al. High Prevalence and Genetic Heterogeneity of Rodent-Borne Bartonella Species on Heixiazi Island, China[J]. Applied and Environmental Microbiology, 2015, 81(23):7981-7992.
Wang J , Lin P , Zhao H , et al. Continuing evolution of canine parvovirus in China: Isolation of novel variants with an Ala5Gly mutation in the VP2 protein.[J]. Infection Genetics & Evolution, 2016, 38:73-78.
Xiang R H , Juan Y , Peng G , et al. Bartonella Species Detected in the Plateau Pikas (Ochotona curzoiae) from Qinghai Plateau in China[J]. Biomedical and Environmental Sciences, 2015, 28(9):674-678.
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