6X BX/ Loading buffer
Referência L0030
Tamanho : 5x1mL
Marca : Canvax Biotech
6X BX/ Loading buffer
Ready-to-use DNA loading dye with Bromophenol Blue and Xylene Cyanol FF for accurate electrophoresis tracking.
6× BX Loading Buffer from Canvax™ is a ready-to-use DNA loading dye containing Bromophenol Blue and Xylene Cyanol FF, specially formulated for accurate visualization and tracking of DNA migration during agarose gel electrophoresis.
The optimized 6× formulation ensures dense sample loading, precise migration fronts, and reproducible separation of DNA fragments. Its compatibility with standard TAE and TBE buffers makes it ideal for routine molecular biology workflows, from PCR product analysis to restriction digestion verification.
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Detailed information:
Advantages & Features
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Dual tracking dyes: Combines Bromophenol Blue and Xylene Cyanol FF for clear migration tracking.
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Ready-to-use solution: No preparation needed—add directly to samples.
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High density: Facilitates accurate and effortless well loading.
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Wide compatibility: Suitable for use with TAE and TBE buffer systems.
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Non-interfering formulation: Safe for downstream gel extraction and enzymatic reactions.
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Stable and reliable: Long shelf-life at ambient temperature without performance loss.
Specifications
- Concentration: 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% Glycerol, 10 mM Tris-HCl (pH 7.6) and 60 mM EDTA.
Includes
– 5 x 1 mL of selected Loading Buffer
Download documentation
Applications
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DNA gel electrophoresis.
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Visualization of DNA migration fronts.
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PCR and restriction digest analysis.
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Agarose gel band tracking.
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Routine molecular biology assays.
Tables & Figures
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Quality Control
- Each lot is tested for consistent dye migration, absence of nucleases, and optical clarity.
Advice
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Mix 1 volume of 6× BX Loading Buffer with 5 volumes of DNA sample.
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Avoid overloading the wells to maintain sharp, defined bands.
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Run gels alongside DNA ladders for accurate fragment tracking.
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Keep tightly closed to prevent evaporation and contamination.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: 4 °C.
Citations
- Ghadamnan, Elmira. Characterization of Tick-Associated Microbiota in Spain Using a Nanopore-based Metabarcoding Approach: Insights into Potential Zoonotic Pathogens. 2023.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
DNA loading buffer 6x, Gel loading dye 6x, DNA sample loading buffer, Electrophoresis loading buffer, Agarose gel loading dye
Also known as:
- Spanish: Buffer de carga ADN 6X, Colorante de carga para gel, Tampón de carga para electroforesis
- French: Tampon de chargement ADN 6X, Colorant de chargement pour gel, Tampon de chargement électrophorèse
- German: DNA-Ladepuffer 6X, Ladefarbstoff für Gel, Elektrophorese-Ladepuffer
- Italian: Tampone di caricamento DNA 6X, Colorante di caricamento per gel, Tampone per elettroforesi
FAQs
Q1: What dyes are used in 6× BX Loading Buffer?
It contains Bromophenol Blue and Xylene Cyanol FF, which migrate near 300 bp and 4 kb respectively in a 1 % agarose gel.
Q2: Is the buffer compatible with DNA recovery kits?
Yes. The formulation does not interfere with gel extraction or downstream enzymatic reactions.
Q3: Can it be used for RNA gels?
Yes, under non-denaturing conditions the buffer performs reliably with RNA samples.