AapCas12b, Active
Referência MBS516659-0,02mg
Tamanho : 0.02mg
Marca : MyBiosource
SDS-PAGE gel image
The purity of AapCas12b, Active was determined to be >80% by densitometry.
Calculated MW: 130.5 kDa
Observed MW: ~128 kDa
The purity of AapCas12b, Active was determined to be >80% by densitometry.
Calculated MW: 130.5 kDa
Observed MW: ~128 kDa
AapCas12b nuclease activity assayed using a fluorogenic substrate and 40nM of enzyme as per the Cas12 Activity Assay Protocol provided below.
AapCas12b Recombinant Protein
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS516659Recombinant full length, tag-free Alicyclobacillus acidiphilus AapCas12b protein expressed in E. coli More details
Product Overview
Online Datasheet in PDFProduct Name
AapCas12b, Recombinant Protein
Full Product Name
AapCas12b, Active
Product Gene Name
AapCas12b recombinant protein
Product Synonym Names
c2c1, CRISPR-associated endonuclease C2c1, Cas12b
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.5, 300mM NaCl, 1mM DTT, 10% glycerol.
Purity/Purification
>80%
Concentration
0.1 ug/ul , 7.66 pmol/ug (lot specific)
Preparation and Storage
Ships on dry ice.
Store at -70 degree C. To avoid repeated handling and multiple freeze/thaw cycles aliquot product into smaller quantities.
Store at -70 degree C. To avoid repeated handling and multiple freeze/thaw cycles aliquot product into smaller quantities.
Scientific Background
CRISPR (clustered regularly interspaced short palindromic repeat) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria. This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development. AapCas12b belongs to the type V CRISPR effector, CRISPR-Cas12b/C2c1, and due to its thermostability, it can be used in a wide range of biomedical applications including loop-mediated isothermal amplification (LAMP), mammalian genome editing, and gene activation.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Product Categories/Family for AapCas12b recombinant protein
CRISPR Associated Proteins
Application Notes for AapCas12b recombinant protein
Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions.
Product References and Citations for AapCas12b recombinant protein
1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89. 2. Lucia C, et al: An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on CRISPR-Cas12. bioRxiv. doi: https://doi.org/10.1101/2020.02.29.971127. 3. Teng F, et al: Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell discovery. 2018, 4(1):1-5.
Other Notes
Small volumes of AapCas12b recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AapCas12b purchase
MBS516659 is a recombinant protein targeting AapCas12b. Also known as AapCas12b, Active. Host: E Coli.
Additional Details
NCBI/Uniprot data below describe general gene information for AapCas12b. It may not necessarily be applicable to this product.
NCBI Accession #
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.