Agaricus bisporus Lectin (ABA/ABL) - Pure

 Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent protein.

Application: Glycobiology, Agglutination, Cell Typing

Recommended Usage: Recommended concentrations from 0.5-10 µg/ml in 1XPBS.  Phosphate buffer 1X, pH 7.4 can be used to reconstitute the lyophilized cake.

0.5-10 µg/ml will agglutinate type A or B cells. 2 µg/ml will agglutinate type O cells. Less than 0.5 µg/ml will agglutinate neuraminidase treated erythrocytes.

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Blotting, Glycobiology, Immunohistochemistry, Immunofluorescence

References

Belur, S., Barkeer, S., Swamy, B. M., Yu, L. G., & Inamdar, S. R. (2019). Investigation of TF-binding lectins from dietary sources and SRL on proliferation and cell cycle progression in human colon HT29 and SW620 cells. Nutrition and cancer, 71(4), “642. https://doi.org/10.1080/01635581.2018.1559940

Ditamo, Y., Rupil, L. L., Sendra, V. G., Nores, G. A., Roth, G. A., & Irazoqui, F. J. (2016). In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus. Food & function, 7(1), “269. https://doi.org/10.1039/c5fo00360a