K2026-500 assays | AK Bioluminescence Cytotoxicity Assay Kit Clinisciences

Background

Cells treatment with the cytotoxic compound may undergo necrosis, in which they lose membrane integrity. Hence cytotoxicity can be detected by the quantification of plasma membrane damage. Adenylate kinase (AK) is a phosphotransferase enzyme that ubiquitously present in all prokaryotic and eukaryotic cells. When damage of the plasma membrane, AK is rapidly released into the cell culture medium.

The AK Bioluminescence Cytotoxicity Assay Kit provides a simple and fast way for the quantification of cytotoxicity based on the measurement of AK involving two chemical reactions. First, AK that is released from the damaged cells converts ADP to ATP. Second, luciferase catalyzes the formation of light from luciferin and ATP, and the light can be detected using a beta counter or luminometer. The assay is highly sensitive and can be fully automatic for high throughput.

Features & Properties

Features	Fast and convenient Simple one-step procedure; takes less than 30 minutes. The assay is highly sensitive and can be fully automatic for high throughput studies.
Shipping	Gel pack
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

AK Detection Reagent (Lyophilized) AK Assay Buffer

AK Bioluminescence Cytotoxicity Assay Kit

Referência K2026-500 assays

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Marca : ApexBio Technology